Experimental methods for mechanically stimulating the cells in vitro

Abstract

细胞力学是组织工程和细胞工程的基础之一 ,在细胞离体培养过程中对细胞施加不同的机械力以研究应力对细胞的影响是细胞力学的一个重要研究领域 ,也是细胞力学的重要研究手段。本文综述了离体培养细胞的力学实验方法 ,根据不同的载荷施加方式可分为 :微吸管吸吮技术、压力载荷法、基底拉伸法和流体剪切法等 ,并指出了各种方法存在的利与弊 Cellular mechanics is a branch of tissue engineering and cellular engineering. As one of the important method, loading different mechanical stimuli to culturing cells in vitro so as to study the influence that the stress has on the cells is one of the important fields of cellular mechanics. This paper reviews the experimental methods for mechanically stimulating the cells in vitro, according to the different loading modalities, the methods can be categorized into micropipette aspiration,compression loading,substrate distention,fluid shear, etc. And it also points out their advantages and disadvantages.