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Title: Robust enzymatic saccharification of a Douglas-fir forest harvest residue by SPORL
Authors: Leu, SY
Zhu, JY
Gleisner, R
Sessions, J
Marrs, G
Issue Date: Dec-2013
Source: Biomass and bioenergy, Dec 2013, v. 59, p. 393–401
Abstract: Forest harvest residues can be a cost-effective feedstock for a biorefinery, but the high lignin content of forest residues is a major barrier for enzymatic sugar production. Sulfite pretreatment to overcome strong recalcitrance of lignocelluloses (SPORL) was applied to a Douglas-fir (Pseudotsuga menziesii (Mirb) Franco var. menziesii) forest residue in a range of sulfite and acid loadings at 165 °C for 75 min with liquid to wood ratio of 3:1. Sodium bisulfite and sulfuric acid charge as mass fraction of oven dry biomass of 12% and 2.21%, respectively, was optimal in terms of enzymatic cellulose saccharification, sugar yield and formation of hydroxymethylfurfural (HMF) and furfural. Enzymatic glucose yield was 345 g kg⁻¹, or equivalent to 82.3% of theoretical at a cellulase (CTec2) dosage of 15 filter paper unit (FPU) per gram of glucan. HMF and furfural formation were low at approximately 2.5 g L⁻¹ each in the pretreatment hydrolyzate. Delignification was important to achieve good cellulose saccharification efficiency, however, approximately 80–90% hemicellulose removal is also required. Substrate enzymatic digestibility (SED) was found to correlate to a combined parameter Z(CHF) of delignification and hemicellulose dissolution well, suggesting that the combined hydrolysis factor (CHF) – a pretreatment severity measure – can be used to predict saccharification of forest residue for scale-up studies to reduce numbers of experiments.
Keywords: Forest harvest residue
Enzymatic hydrolysis/saccharification
Pretreatment severity
Publisher: Elsevier
Journal: Biomass and bioenergy 
ISSN: 0961-9534
DOI: 10.1016/j.biombioe.2013.08.014
Rights: NOTICE: this is the author’s version of a work that was accepted for publication in Biomass and Bioenergy. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomass and Bioenergy, VOL 59, December 2013, DOI: 10.1016/j.biombioe.2013.08.014
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