Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/6249
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dc.contributorDepartment of Applied Biology and Chemical Technology-
dc.creatorWang, Nen_US
dc.creatorWan, JBen_US
dc.creatorChan, SWen_US
dc.creatorDeng, YHen_US
dc.creatorYu, Nen_US
dc.creatorZhang, QWen_US
dc.creatorWang, YTen_US
dc.creatorLee, SMYen_US
dc.date.accessioned2014-12-11T08:23:24Z-
dc.date.available2014-12-11T08:23:24Z-
dc.identifier.issn1749-8546en_US
dc.identifier.urihttp://hdl.handle.net/10397/6249-
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.rights© 2011 Wang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.titleComparative study on saponin fractions from Panax notoginseng inhibiting inflammation-induced endothelial adhesion molecule expression and monocyte adhesionen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.volume6en_US
dc.identifier.doi10.1186/1749-8546-6-37en_US
dcterms.abstractBackground: Panax notoginseng is commonly used for the treatment of cardiovascular diseases in China. The present study investigates the effects of three different saponin fractions (ie total saponins, PNS; protopanaxadiol-type saponin, PDS; and protopanaxatriol-type saponin, PTS) and two major individual ingredients (ie ginsenoside Rg1 and Rb1) from P. notoginseng on the endothelial inflammatory response in vitro and in vivo.-
dcterms.abstractMethods: Recombinant human tumor necrosis factor-α (TNF-α) was added to the culture medium of human coronary artery endothelial cells (HCAECs) to induce an inflammatory response. A cell adhesion assay was used to determine the effect of the P. notoginseng saponin fractions on endothelial-monocyte interaction. The cell adhesion molecule (CAMs) expression, including ICAM-1 and VCAM-1, in the protein level on the surface of endothelial cells were measured by cellular ELISA. CAMs expression in mRNA level was also assayed by qRT-PCR in the HCAECs and the aorta of rat fed with high cholesterol diet (HCD). Western blotting was used to detect effect of the saponin fractions on CAMs protein expression in HCAECs. In addition, nuclear translocation of p65, a surrogate marker for NF-κB activation, was measured by immunostaining.-
dcterms.abstractResult: Three saponin fractions and two individual ginsenosides exhibited the inhibitory effects on monocyte adhesion on TNF-α-activated HCAECs and expression of ICAM-1 and VCAM-1 at both mRNA and protein levels in vitro. The saponin fractions exhibited a similar trend of the inhibitory effects on the mRNA expression of CAMs in the aorta of HCD-fed rat in vivo. These inhibitory effect of saponin fractions maybe attribute partially to the suppression of the TNF-α-induced NF-κB activation.-
dcterms.abstractConclusion: Our data demonstrate that saponin fractions (ie PNS, PDS and PTS) and major individual ginsenosides (ie Rg1 and Rb1) have potential anti-atherogenic effects. Among the tested saponin fractions, PDS is the most potent saponin fraction against TNF-α-induced monocyte adhesion as well as the expression of adhesion molecules in vitro and in vivo.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationChinese medicine, 13 Oct 2011, v. 6, 37, p.1-12en_US
dcterms.isPartOfChinese Medicineen_US
dcterms.issued2011-10-13-
dc.identifier.isiWOS:000208722100037-
dc.identifier.scopus2-s2.0-80053907452-
dc.identifier.pmid21995855-
dc.identifier.rosgroupidr58582-
dc.description.ros2011-2012 > Academic research: refereed > Publication in refereed journal-
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_IR/PIRAen_US
dc.description.pubStatusPublisheden_US
dc.description.oaCategoryCCen_US
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