Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/5582
| Title: | Covalently immobilized biomolecule gradient on hydrogel surface using a gradient generating microfluidic device for a quantitative mesenchymal stem cell study | Authors: | Liu, Z Xiao, L Xu, B Zhang, Y Mak, AFT Li, Y Man, WY Yang, M |
Issue Date: | Jun-2012 | Source: | Biomicrofluidics, June 2012, v. 6, no. 2, 024111, p. 1-12 | Abstract: | Precisely controlling the spatial distribution of biomolecules on biomaterial surface is important for directing cellular activities in the controlled cell microenvironment. This paper describes a polydimethylsiloxane (PDMS) gradient-generating microfluidic device to immobilize the gradient of cellular adhesive Arg-Gly-Asp (RGD) peptide on poly (ethylene glycol) (PEG) hydrogel. Hydrogels are formed by exposing the mixture of PEG diacrylate (PEGDA), acryloyl-PEG-RGD, and photo-initiator with ultraviolet light. The microfluidic chip was simulated by a fluid dynamic model for the biomolecule diffusion process and gradient generation. PEG hydrogel covalently immobilized with RGD peptide gradient was fabricated in this microfluidic device by photo-polymerization. Bone marrow derived rat mesenchymal stem cells (MSCs) were then cultured on the surface of RGD gradient PEG hydrogel. Cell adhesion of rat MSCs on PEG hydrogel with various RGD gradients were then qualitatively and quantitatively analyzed by immunostaining method. MSCs cultured on PEG hydrogel surface with RGD gradient showed a grated fashion for cell adhesion and spreading that was proportional to RGD concentration. It was also found that 0.107–0.143 mM was the critical RGD concentration range for MSCs maximum adhesion on PEG hydrogel. | Keywords: | Adhesion Biochemistry Biodiffusion Biological techniques BioMEMS Bone Cellular biophysics Hydrogels Lab-on-a-chip Microfabrication Microfluidics Molecular biophysics Polymerisation |
Publisher: | American Institute of Physics | Journal: | Biomicrofluidics | ISSN: | 1932-1058 (online) | DOI: | 10.1063/1.4704522 | Rights: | © 2012 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Z. Liu et al. Biomicrofluidics 6, 024111 (2012) and may be found at http://link.aip.org/link/?bmf/6/024111 |
| Appears in Collections: | Journal/Magazine Article |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Liu_Covalently_Immobilized_Biomolecule.pdf | 3.24 MB | Adobe PDF | View/Open |
Access
View full-text via PolyU eLinks 
Page views
152
Last Week
1
1
Last month
Citations as of Sep 24, 2023
Downloads
153
Citations as of Sep 24, 2023
SCOPUSTM
Citations
35
Last Week
1
1
Last month
0
0
Citations as of Sep 28, 2023
WEB OF SCIENCETM
Citations
32
Last Week
2
2
Last month
0
0
Citations as of Sep 28, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.