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Title: | Regulatory effect of puerarin on lipid profile in hypercholesterolemic rats | Other Title: | 葛根素对膳食诱导的高胆固醇血症大鼠的血脂调节作用 | Authors: | Chen, SL Yan, LP Chen, SH Chen, WM Ma, XJ Xu, HX |
Issue Date: | 2006 | Source: | 中国临床药理学与治疗学 (Chinese Journal of clinical pharmacology and therapeutics), 2006, v. 11, no. 5, p. 574-577 | Abstract: | 目的:观察葛根素对膳食诱导的高胆固 醇血症大鼠的血脂调节作用,并对其机制进行探索。方法:SD大鼠随机分为3组,正常组、模型组和葛根素组。模型组和葛根素组均给予富含胆固醇的饲料4周, 葛根素组每天灌喂葛根素溶液(300mg·kg-1·d-1)。检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-c)和低密度 脂蛋白胆固醇(LDL-c)的含量。荧光定量RT-PCR分析羟基-3-甲基戊二酰CoA还原酶(HMGR),羊毛固醇14α-去甲基酶 (CYP51),7α-羟化酶(CYP7A1)的mRNA转录水平。结果:葛根素显著降低高胆固醇膳食引起的血清胆固醇的升高,降低动脉粥样硬化指数,上 调CYP7A1的mRNA转录水平,对HMGR和CYP51的转录水平没有影响。结论:这些数据表明葛根素能够降低血清胆固醇,减低动脉粥样硬化发生危 险。其降低胆固醇的作用可能是通过促进胆固醇转化为胆酸实现的。 AIM: To investigate the regulatory effect of puerarin on lipid profile in diet-induced hypercholesterolemic rats and elucidate the mechanisms involved. METHODS: SD rats were randomly divided into the control group, hypercholesterolemia group and puerarin-treated group. The hypercholesterolemia group and puerarin-treated group were given diet rich in cholesterol. In the meantime, the puerarin-treated group was orally administrated with puerarin (300 mg·kg~ -1 ·d~ -1 ). The lipid profile was investigated by the content of serum TC, TG, HDL-c and LDL-c. The mRNA levels of 7α-hydroxylase (CYP7A1), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and lanosterol 14α-demethylase (CYP51) were analyzed by real time RT-PCR. RESULTS: Puerarin markedly attenuated the increased serum total cholesterol induced by hypercholesterolemic diet. It caused a significant reduction in the atherogenic index. Expression of mRNA of CYP7A1 was significantly enhanced but not for those of HMGR and CYP51. CONCLUSION: These data indicate that puerarin can reduce the atherogenic properties of dietary cholesterol in rats. Its hypocholesterolemic function may be due to promoting the conversion of cholesterol into bile acids. |
Keywords: | Hypercholesterolemia Serum lipids levels Atherosclerosis 3-hydroxy-3-methylglutaryl-coa reductase Lanosterol 14α-demethylase 7α-hydroxylase |
Publisher: | 中国学术期刊(光盘版)电子杂志社 | Journal: | 中国临床药理学与治疗学 (Chinese Journal of clinical pharmacology and therapeutics) | ISSN: | 1009-2501 | Rights: | © 2006 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research use. © 2006 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。 |
Appears in Collections: | Journal/Magazine Article |
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