Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/2374
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Wu, J | - |
dc.creator | Li, Q | - |
dc.creator | Wu, M | - |
dc.creator | Guo, D | - |
dc.creator | Chen, HL | - |
dc.creator | Chen, S | - |
dc.creator | Seto, SW | - |
dc.creator | Au, ALS | - |
dc.creator | Poon, CCW | - |
dc.creator | Leung, GPH | - |
dc.creator | Lee, SMY | - |
dc.creator | Kwan, YW | - |
dc.creator | Chan, SW | - |
dc.date.accessioned | 2014-12-11T08:28:55Z | - |
dc.date.available | 2014-12-11T08:28:55Z | - |
dc.identifier.issn | 0955-2863 | - |
dc.identifier.uri | http://hdl.handle.net/10397/2374 | - |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Journal of Nutritional Biochemistry © 2010 Elsevier Inc. The journal web site is located at http://www.sciencedirect.com. | en_US |
dc.subject | Formononetin | en_US |
dc.subject | Nitric oxide | en_US |
dc.subject | Vasorelaxation | en_US |
dc.subject | BK[sub ca] and K[sub ATP] channels | en_US |
dc.title | Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.description.otherinformation | Author name used in this publication: Jian-Hong Wu | en_US |
dc.description.otherinformation | Author name used in this publication: Min-Yi Wu | en_US |
dc.description.otherinformation | Author name used in this publication: De-Jian Guo | en_US |
dc.description.otherinformation | Author name used in this publication: Shi-Lin Chen | en_US |
dc.description.otherinformation | Author name used in this publication: Alice L. S. Au | en_US |
dc.description.otherinformation | Author name used in this publication: Christina C. W. Poon | en_US |
dc.identifier.spage | 613 | - |
dc.identifier.epage | 620 | - |
dc.identifier.volume | 21 | - |
dc.identifier.issue | 7 | - |
dc.identifier.doi | 10.1016/j.jnutbio.2009.03.010 | - |
dcterms.abstract | We evaluated the vasorelaxation effects of formononetin, an isoflavone/phytoestrogen found abundantly in Astragalus mongholicus Bunge, on rat isolated aorta and the underlying mechanisms involved. Cumulative administration of formononetin, genistein, daidzein and biochanin A relaxed phenylephrine-preconstricted aorta. Formononetin and biochanin A caused a similar magnitude of relaxation whereas daidzein was least potent. Mechanical removal of endothelium, L-NAME (100 μM) and methylene blue (10 μM) suppressed formononetin-induced relaxation. Formononetin increased endothelial nitric oxide (NO) synthase (eNOS), but not inducible NO synthase, activity with an up-regulation of eNOS mRNA and p-eNOS[sup Ser]¹¹ ⁷ ⁷ protein expression. In endothelium-denuded preparations, formononetin-induced vasorelaxation was significantly reduced by glibenclamide (3 μM) and iberiotoxin (100 nM), and a combination of glibenclamide (3 μM) plus iberiotoxin (100 nM) abolished the relaxation. In contrast, formononetin-elicited endothelium-independent relaxation was not altered by ICI 182,780 (10 μM, an estrogen receptor (ERα/ERβ) antagonist) or mifepristone (10 μM, a progesterone receptor antagonist). In single aortic smooth muscle cells, formononetin caused opening of iberiotoxin-sensitive Ca²⁺-activated K⁺ (BK[sub ca]) channels and glibenclamide-sensitive adenosine triphosphate (ATP)- dependent K⁺ (K[sub ATP]) channels. Thus, our results suggest that formononetin caused vascular relaxation via endothelium/NO-dependent mechanism and endothelium-independent mechanism which involves the activation of BK[sub ca] and K[sub ATP] channels. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Journal of nutritional biochemistry, July 2010, v. 21, no. 7, p. 613-620 | - |
dcterms.isPartOf | Journal of nutritional biochemistry | - |
dcterms.issued | 2010-07 | - |
dc.identifier.isi | WOS:000279363200007 | - |
dc.identifier.scopus | 2-s2.0-77953812949 | - |
dc.identifier.pmid | 19570671 | - |
dc.identifier.rosgroupid | r54014 | - |
dc.description.ros | 2010-2011 > Academic research: refereed > Publication in refereed journal | - |
dc.description.oa | Accepted Manuscript | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
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Formononetin (Final).pdf | Pre-published version | 900.67 kB | Adobe PDF | View/Open |
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