Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/13775
DC Field | Value | Language |
---|---|---|
dc.contributor | School of Optometry | - |
dc.creator | Li, SY | - |
dc.creator | Fung, FKC | - |
dc.creator | Fu, ZJ | - |
dc.creator | Wong, D | - |
dc.creator | Chan, HHL | - |
dc.creator | Lo, ACY | - |
dc.date.accessioned | 2015-07-14T01:30:03Z | - |
dc.date.available | 2015-07-14T01:30:03Z | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | http://hdl.handle.net/10397/13775 | - |
dc.language.iso | en | en_US |
dc.publisher | Association for Research in Vision and Ophthalmology | en_US |
dc.rights | Posted with permission of the publisher | en_US |
dc.rights | Copyright 2012 The Association for Research in Vision and Ophthalmology, Inc. | en_US |
dc.title | Anti-inflammatory effects of lutein in retinal ischemic/hypoxic injury : in vivo and in vitro studies | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 5976 | - |
dc.identifier.epage | 5984 | - |
dc.identifier.volume | 53 | - |
dc.identifier.issue | 10 | - |
dc.identifier.doi | 10.1167/iovs.12-10007 | - |
dcterms.abstract | PURPOSE. Lutein protects retinal neurons by its anti-oxidative and anti-apoptotic properties in ischemia/reperfusion (I/R) injury while its anti-inflammatory effects remain unknown. As Muller cells play a critical role in retinal inflammation, the effect of lutein on Muller cells was investigated in a murine model of I/R injury and a culture model of hypoxic damage. METHODS. Unilateral retinal I/R was induced by a blockade of internal carotid artery using the intraluminal method in mice. Ischemia was maintained for 2 hours followed by 22 hours of reperfusion, during which either lutein (0.2 mg/kg) or vehicle was administered. Flash electroretinogram (flash ERG) and glial fibrillary acidic protein (GFAP) activation were assessed. Lutein's effect on Muller cells was further evaluated in immortalized rat Muller cells (rMC-1) challenged with cobalt chloride-induced hypoxia. Levels of IL-1 beta, cyclooxygenase-2 (Cox-2), TNF alpha, and nuclear factor-NF-kappa-B (NF-kappa B) were examined by Western blot analysis. RESULTS. Lutein treatment minimized deterioration of b-wave/a-wave ratio and oscillatory potentials as well as inhibited up-regulation of GFAP in retinal I/R injury. In cultured Muller cells, lutein treatment increased cell viability and reduced level of nuclear NF-kappa B, IL-1 beta, and Cox-2, but not TNF alpha after hypoxic injury. CONCLUSIONS. Reduced gliosis in I/R retina was observed with lutein treatment, which may contribute to preserved retinal function. Less production of pro-inflammatory factors from Muller cells suggested an anti-inflammatory role of lutein in retinal ischemic/hypoxic injury. Together with our previous studies, our results suggest that lutein protected the retina from ischemic/hypoxic damage by its anti-oxidative, antiapoptotic, and anti-inflammatory properties. | - |
dcterms.accessRights | open access | - |
dcterms.bibliographicCitation | Investigative ophthalmology and visual science, 2012, v. 53, no. 10, p. 5976-5984 | - |
dcterms.isPartOf | Investigative ophthalmology and visual science | - |
dcterms.issued | 2012 | - |
dc.identifier.isi | WOS:000309526200001 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.rosgroupid | r65219 | - |
dc.description.ros | 2012-2013 > Academic research: refereed > Publication in refereed journal | - |
dc.description.oa | Version of Record | - |
dc.identifier.FolderNumber | a0780-n08 | - |
dc.identifier.SubFormID | 1632 | - |
dc.description.fundingSource | RGC | - |
dc.description.fundingText | HKU 7732/10M | - |
dc.description.pubStatus | Published | - |
dc.description.oaCategory | Publisher permission | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
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1632_Li_et_al_IOVS_2012.pdf | 663.62 kB | Adobe PDF | View/Open |
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