Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/107306
Title: | Oleanolic acid exerts bone anabolic effects via activation of osteoblastic 25-hydroxyvitamin D 1-alpha hydroxylase | Authors: | Yu, WX Poon, CCW Zhou, LP Wong, KY Cao, SS Lam, CY Lee, WYW Wong, MS |
Issue Date: | Apr-2024 | Source: | Biomedicine and pharmacotherapy, Apr. 2024, v. 173, 116402 | Abstract: | Oleanolic acid (OA) is previously shown to exert bone protective effects in aged animals. However, its role in regulating osteoblastic vitamin D bioactivation, which is one of major causes of age-related bone loss, remains unclear. Our results revealed that treatment of OA significantly increased skeletal CYP27B1 expression and circulating 1,25(OH)2D3 in ovariectomized mice (p <0.01). Moreover, OA upregulated CYP27B1 protein expression and activity, as well as the vitamin D-responsive bone markers alkaline phosphatase (ALP) activity and osteopontin (OPN) protein expression, in human osteoblast-like MG-63 cells (p<0.05). CYP27B1 expression increased along with the osteoblastic differentiation of human bone marrow derived mesenchymal stem cells (hMSCs). CYP27B1 expression and cellular 1,25(OH)2D3 production were further potentiated by OA in cells at mature osteogenic stages. Notably, our study suggested that the osteogenic actions of OA were CYP27B1 dependent. In summary, the bone protective effects of OA were associated with the induction of CYP27B1 activity and expression in bone tissues and osteoblastic lineages. Hence, OA might be a potential approach for management of age-related bone loss. | Keywords: | 25-Hydroxyvitamin D3 1-alpha-hydroxylase Aging Oleanolic acid Osteoporosis Vitamin D |
Publisher: | Elsevier Masson | Journal: | Biomedicine and pharmacotherapy | ISSN: | 0753-3322 | EISSN: | 1950-6007 | DOI: | 10.1016/j.biopha.2024.116402 | Rights: | © 2024 The Authors. Published by Elsevier Masson SAS. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/). The following publication Yu, W.-X., Poon, C. C.-W., Zhou, L.-P., Wong, K.-Y., Cao, S.-S., Lam, C.-Y., Lee, W. Y.-w., & Wong, M.-S. (2024). Oleanolic acid exerts bone anabolic effects via activation of osteoblastic 25-hydroxyvitamin D 1-alpha hydroxylase. Biomedicine & Pharmacotherapy, 173, 116402 is available at https://doi.org/10.1016/j.biopha.2024.116402. |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
1-s2.0-S0753332224002865-main.pdf | 2 MB | Adobe PDF | View/Open |
Page views
6
Citations as of Jun 30, 2024
Downloads
4
Citations as of Jun 30, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.