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Title: | Cornea-SELEX for aptamers targeting the surface of eyes and liposomal drug delivery | Authors: | Wong, KY Liu, Y Wong, MS Liu, J |
Issue Date: | 2024 | Source: | Exploration, First published: 09 February 2024, Early View, https://doi.org/10.1002/EXP.20230008 | Abstract: | Cornea is the major barrier to drug delivery to the eye, which results in low bioavailability and poor efficacy of topical eye treatment. In this work, we first select cornea-binding aptamers using tissue-SELEX on pig cornea. The top two abundant aptamers, Cornea-S1 and Cornea-S2, could bind to pig cornea, and their Kd values to human corneal epithelial cells (HCECs) were 361 and 174 nм, respectively. Aptamer-functionalized liposomes loaded with cyclosporine A (CsA) were developed as a treatment for dry eye diseases. The Kd of Cornea-S1- or Cornea-S2-functionalized liposomes reduces to 1.2 and 15.1 nм, respectively, due to polyvalent binding. In HCECs, Cornea-S1 or Cornea-S2 enhanced liposome uptake within 15 min and extended retention to 24 h. Aptamer CsA liposomes achieved similar anti-inflammatory and tight junction modulation effects with ten times less CsA than a free drug. In a rabbit dry eye disease model, Cornea-S1 CsA liposomes demonstrated equivalence in sustaining corneal integrity and tear break-up time when compared to commercial CsA eye drops while utilizing a lower dosage of CsA. The aptamers obtained from cornea-SELEX can serve as a general ligand for ocular drug delivery, suggesting a promising avenue for the treatment of various eye diseases and even other diseases. | Keywords: | Aptamers Cornea Tissue-SELEX |
Publisher: | John Wiley & Sons, Inc. | Journal: | Exploration | ISSN: | 2766-8509 | EISSN: | 2766-2098 | DOI: | 10.1002/EXP.20230008 | Rights: | © 2024 The Authors. Exploration published by Henan University and John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the originalwork is properly cited. The following publication K.-Y. Wong, Y. Liu, M.-S. Wong, J. Liu, Exploration 2024, 20230008 is available at https://doi.org/10.1002/EXP.20230008. |
Appears in Collections: | Journal/Magazine Article |
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Wong_Cornea-SELEX_Aptamers_Targeting.pdf | 2.49 MB | Adobe PDF | View/Open |
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