Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/107303
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Food Science and Nutrition | - |
dc.contributor | Research Centre for Chinese Medicine Innovation | - |
dc.contributor | School of Optometry | - |
dc.creator | Wong, KY | - |
dc.creator | Kong, TH | - |
dc.creator | Poon, CCW | - |
dc.creator | Yu, W | - |
dc.creator | Zhou, L | - |
dc.creator | Wong, MS | - |
dc.date.accessioned | 2024-06-13T07:07:53Z | - |
dc.date.available | 2024-06-13T07:07:53Z | - |
dc.identifier.issn | 0951-418X | - |
dc.identifier.uri | http://hdl.handle.net/10397/107303 | - |
dc.language.iso | en | en_US |
dc.publisher | John Wiley & Sons Ltd. | en_US |
dc.subject | Estradiol | en_US |
dc.subject | Estrogen receptors | en_US |
dc.subject | Icariin | en_US |
dc.subject | Natural product | en_US |
dc.subject | Osteoblast | en_US |
dc.subject | Receptor crosstalk | en_US |
dc.title | Icariin, a phytoestrogen, exerts rapid estrogenic actions through crosstalk of estrogen receptors in osteoblasts | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 4706 | - |
dc.identifier.epage | 4721 | - |
dc.identifier.volume | 37 | - |
dc.identifier.issue | 10 | - |
dc.identifier.doi | 10.1002/ptr.7939 | - |
dcterms.abstract | Icariin, a flavonoid glycoside derived from Epimedium brevicornum Maxim, exerts bone protective effects via estrogen receptors (ERs). This study aimed to investigate the role of ER-α66, ER-α36, and GPER in bone metabolism in osteoblasts following treatment with icariin. Human osteoblastic MG-63 cells and osteoblast-specific ER-α66 knockout mice were employed. The ERs crosstalk in the estrogenic action of icariin was evaluated in ER-α66-negative human embryonic kidney HEK293 cells. Icariin, like E2, regulated ER-α36 and GPER protein expression in osteoblasts by downregulating them and upregulating ER-α66. ER-α36 and GPER suppressed the actions of icariin and E2 in bone metabolism. However, the in vivo administration of E2 (2 mg/kg/day) or icariin (300 mg/kg/day) restored bone conditions in KO osteoblasts. ER-α36 and GPER expression increased significantly and rapidly activated and translocated in KO osteoblasts after treatment with E2 or icariin. ER-α36 overexpression in KO osteoblasts further promoted the OPG/RANKL ratio induced by E2 or icariin treatment. This study showed icariin and E2 elicit rapid estrogenic responses in bone through recruiting ER-α66, ER-α36, and GPER. Notably, in osteoblasts lacking ER-α66, ER-α36, and GPER mediate the estrogenic effects of icariin and E2, while in intact osteoblasts, ER-α36 and GPER act as negative regulators of ER-α66. | - |
dcterms.accessRights | embargoed access | en_US |
dcterms.bibliographicCitation | Phytotherapy research, Oct. 2023, v. 37, no. 10, p. 4706-4721 | - |
dcterms.isPartOf | Phytotherapy research | - |
dcterms.issued | 2023-10 | - |
dc.identifier.scopus | 2-s2.0-85164600621 | - |
dc.identifier.eissn | 1099-1573 | - |
dc.description.validate | 202406 bcch | - |
dc.identifier.FolderNumber | a2811 | en_US |
dc.identifier.SubFormID | 48440 | en_US |
dc.description.fundingSource | RGC | en_US |
dc.description.pubStatus | Published | en_US |
dc.date.embargo | 2024-10-31 | en_US |
dc.description.oaCategory | Green (AAM) | en_US |
Appears in Collections: | Journal/Magazine Article |
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