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http://hdl.handle.net/10397/106531
Title: | Characterization of a honeycomb-like scaffold with dielectrophoresis-based patterning for tissue engineering | Authors: | Huan, Z Chu, HK Yang, J Sun, D |
Issue Date: | Apr-2017 | Source: | IEEE transactions on biomedical engineering, Apr. 2017, v. 64, no. 4, p. 755-764 | Abstract: | Objective: Seeding and patterning of cells with an engineered scaffold is a critical process in artificial tissue construction and regeneration. To date, many engineered scaffolds exhibit simple intrinsic designs, which fail to mimic the geometrical complexity of native tissues. In this study, a novel scaffold that can automatically seed cells into multilayer honeycomb patterns for bone tissue engineering application was designed and examined. Methods: The scaffold incorporated dielectrophoresis for noncontact manipulation of cells and intrinsic honeycomb architectures were integrated in each scaffold layer. When a voltage was supplied to the stacked scaffold layers, three-dimensional electric fields were generated, thereby manipulating cells to form into honeycomb-like cellular patterns for subsequent culture. Results: The biocompatibility of the scaffold material was confirmed through the cell viability test. Experiments were conducted to evaluate the cell viability during DEP patterning at different voltage amplitudes, frequencies, and manipulating time. Three different mammalian cells were examined and the effects of the cell size and the cell concentration on the resultant cellular patterns were evaluated. Conclusion: Results showed that the proposed scaffold structure was able to construct multilayer honeycomb cellular patterns in a manner similar to the natural tissue. Significance: This honeycomb-like scaffold and the dielectrophoresis-based patterning technique examined in this study could provide the field with a promising tool to enhance seeding and patterning of a wide range of cells for the development of high-quality artificial tissues. | Keywords: | Cell patterning Cell viability Dielectrophoresis Scaffold |
Publisher: | Institute of Electrical and Electronics Engineers | Journal: | IEEE transactions on biomedical engineering | ISSN: | 0018-9294 | EISSN: | 1558-2531 | DOI: | 10.1109/TBME.2016.2574932 | Rights: | © 2017 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. The following publication Z. Huan, H. K. Chu, J. Yang and D. Sun, "Characterization of a Honeycomb-Like Scaffold With Dielectrophoresis-Based Patterning for Tissue Engineering," in IEEE Transactions on Biomedical Engineering, vol. 64, no. 4, pp. 755-764, April 2017 is available at https://doi.org/10.1109/TBME.2016.2574932. |
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Chu_Characterization_Honeycomb-Like_Scaffold.pdf | Pre-Published version | 988.57 kB | Adobe PDF | View/Open |
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