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Title: | Human placental extract suppresses mast cell activation and induces mast cell apoptosis | Authors: | Wu, TQ He, JJ Yan, SR Li, J Chen, K Zhang, DS Cheng, ML Zou, X Fang, Y |
Issue Date: | 2023 | Source: | Allergy asthma and clinical immunology, 2023, v. 19, 98 | Abstract: | Background Human placental extract (HPE) has been documented to facilitate the healing of certain disorders including allergy. However, the effects of HPE on the functionality of mast cells, a critical cell type in allergic diseases, have not been reported.Methods To investigate the effects of HPE on the regulation of allergy with respect to the biological functions of mast cells, the mast cell line C57 or HMC-1 cells were treated with HPE followed by the assessment of cell proliferation, apoptosis, activation, chemotaxis and phagocytosis. Mouse peritoneal mast cells were also investigated for their responses to induction of apoptosis by HPE in vivo. Furthermore, the effect of HPE on mast cell degranulation was confirmed using the passive cutaneous anaphylaxis (PCA) assay, an acute allergy model.Results HPE was capable of suppressing mast cell proliferation and inducing mast cell apoptosis. Mast cell degranulation in response to compound 48/80- or anti-DNP IgE and DNP-mediated activation was suppressed. In addition, treatment with HPE compromised the production of cytokines by mast cells and cell chemotaxis. These observations were consistent with the dampened passive cutaneous anaphylaxis (PCA) assay following treatment with HPE.Conclusion This study revealed a suppressive effect of HPE on overall mast cell activities, suggesting a potential regulatory role of HPE on the alleviation of allergic diseases through mast cells. | Keywords: | Mast cell Allergy Human placental extract Passive cutaneous anaphylaxis Mast cell activation Degranulation |
Publisher: | BioMed Central Ltd. | Journal: | Allergy asthma and clinical immunology | EISSN: | 1710-1492 | DOI: | 10.1186/s13223-023-00850-y | Rights: | © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. The following publication Wu, T., He, J., Yan, S. et al. Human placental extract suppresses mast cell activation and induces mast cell apoptosis. Allergy Asthma Clin Immunol 19, 98 (2023) is available at https://dx.doi.org/10.1186/s13223-023-00850-y. |
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