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http://hdl.handle.net/10397/105338
Title: | Enzyme-assisted nucleic acid amplification in molecular diagnosis : a review | Authors: | Wang, M Liu, H Ren, J Huang, Y Deng, Y Liu, Y Chen, Z Chow, FWN Leung, PHM Li, S |
Issue Date: | Feb-2023 | Source: | Biosensors, Feb. 2023, v. 13, no. 2, 160 | Abstract: | Infectious diseases and tumors have become the biggest medical challenges in the 21st century. They are driven by multiple factors such as population growth, aging, climate change, genetic predispositions and more. Nucleic acid amplification technologies (NAATs) are used for rapid and accurate diagnostic testing, providing critical information in order to facilitate better follow-up treatment and prognosis. NAATs are widely used due their high sensitivity, specificity, rapid amplification and detection. It should be noted that different NAATs can be selected according to different environments and research fields; for example, isothermal amplification with a simple operation can be preferred in developing countries or resource-poor areas. In the field of translational medicine, CRISPR has shown great prospects. The core component of NAAT lies in the activity of different enzymes. As the most critical material of nucleic acid amplification, the key role of the enzyme is self-evident, playing the upmost important role in molecular diagnosis. In this review, several common enzymes used in NAATs are compared and described in detail. Furthermore, we summarize both the advances and common issues of NAATs in clinical application. | Keywords: | Clinical application CRISPR-Cas Enzymes Isothermal amplification Nucleic acid amplification technology Polymerase chain reaction |
Publisher: | MDPI AG | Journal: | Biosensors | EISSN: | 2079-6374 | DOI: | 10.3390/bios13020160 | Rights: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). The following publication Wang M, Liu H, Ren J, Huang Y, Deng Y, Liu Y, Chen Z, Chow FW-N, Leung PH-M, Li S. Enzyme-Assisted Nucleic Acid Amplification in Molecular Diagnosis: A Review. Biosensors. 2023; 13(2):160 is available at https://doi.org/10.3390/bios13020160. |
Appears in Collections: | Journal/Magazine Article |
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