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http://hdl.handle.net/10397/91056
| Title: | A hybrid plasmid formed by recombination of a virulence plasmid and a resistance plasmid in Klebsiella pneumoniae | Authors: | Xie, MM Dong, N Chen, KC Yang, XM Ye, LW Chan, EWC Zhang, R Chen, S |
Issue Date: | Dec-2020 | Source: | Journal of global antimicrobial resistance, Dec. 2020, v. 23, p. 466-470 | Abstract: | Objective: The emergence of multidrug-resistant (MDR) and hypervirulent Klebsiella pneumoniae (hvKP) facilitates simultaneous dissemination of virulence and resistance in a single event, which poses serious threat to public health. Methods: This study characterized the multidrug-resistant and moderately virulent ST11 K64 K. pneumoniae strain HB25-1 from a clinical case with microbiological and genomic approaches. Plasmids from strain HB25-1 were subjected to whole plasmid sequencing using both the Illumina NextSeq 500 sequencing platform and Nanopore MinION sequencer platforms. Klebsiella pneumoniae HB25-1 was subjected to a conjugation experiment and Galleria mellonella infection model to evaluate the transmission and virulence potential. Results: We report the emergence of an STII, serotype K64 K. pneumoniae isolate, which is resistant to third-generation cephalosporin and exhibited a moderate level of virulence. WGS revealed that this strain harboured a plasmid, pHB25-1, which carried multidrug resistance genes (bla(DHA)(-1), qnrB4, dfrA12, aadA2, sul1, aac(3)-lld, bla(TEM-1), mph(E)) and virulence-encoding genes (the regulator of mucoid phenotype A gene rmpA2 and the aerobactin gene cluster iutAiucABCD). Genomic analysis indicated that pHB25-1 was formed through co-integration of structural regions located in two different plasmids, enabling it to encode both resistance and virulent phenotypes. Conclusion: Findings in this study provide evidence of active plasmid evolution in K. pneumoniae and suggest that surveillance of multidrug-resistant and hypervirulent K. pneumoniae is urgently needed. |
Keywords: | Klebsiella pneumoniae Multidrug resistance Plasmid Recombination |
Publisher: | Elsevier | Journal: | Journal of global antimicrobial resistance | ISSN: | 2213-7165 | DOI: | 10.1016/j.jgar.2020.10.018 | Rights: | © 2020 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). The following publication K.M.Faridul Hasan, Haona Wang, Sakil Mahmud, Md Anwar Jahid, Moyinul Islam, Wangbao Jin, Cao Genyang, Colorful and antibacterial nylon fabric via in-situ biosynthesis of chitosan mediated nanosilver, Journal of Materials Research and Technology, Volume 9, Issue 6, 2020, Pages 16135-16145, ISSN 2238-7854, https://doi.org/10.1016/j.jmrt.2020.11.056 is available at (https://www.sciencedirect.com/science/article/pii/S2238785420320226) |
| Appears in Collections: | Journal/Magazine Article |
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| Chan_hybrid_plasmid_formed.pdf | 1.07 MB | Adobe PDF | View/Open |
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