Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/96070
DC Field | Value | Language |
---|---|---|
dc.contributor | School of Optometry | en_US |
dc.contributor | School of Optometry | en_US |
dc.contributor | Research Centre for SHARP Vision | en_US |
dc.creator | Lam, CHI | en_US |
dc.creator | Cheung, JKW | en_US |
dc.creator | TSE, DYY | en_US |
dc.creator | Lam, TC | en_US |
dc.date.accessioned | 2022-11-07T02:13:26Z | - |
dc.date.available | 2022-11-07T02:13:26Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/96070 | - |
dc.language.iso | en | en_US |
dc.publisher | Molecular Diversity Preservation International (MDPI) | en_US |
dc.rights | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | en_US |
dc.rights | The following publication Lam, C.H.-I.; Cheung, J.K.-W.; Tse, D.Y.-Y.; Lam, T.C. Proteomic Profiling Revealed Mitochondrial Dysfunction in Photoreceptor Cells under Hyperglycemia. Int. J. Mol. Sci. 2022, 23, 13366 is available at https://doi.org/10.3390/ijms232113366. | en_US |
dc.subject | Diabetic retinopathy | en_US |
dc.subject | SWATH-MS | en_US |
dc.subject | Mitochondrial dysfunction | en_US |
dc.title | Proteomic profiling revealed mitochondrial dysfunction in photoreceptor cells under hyperglycemia | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.volume | 23 | en_US |
dc.identifier.issue | 21 | en_US |
dc.identifier.doi | 10.3390/ijms232113366 | en_US |
dcterms.abstract | Diabetic retinopathy (DR) was identified as a leading cause of blindness and vision impairment in 2020. In addition to vasculopathy, DR has been found to involve retinal neurons, including amacrine cells and retinal ganglion cells. Despite possessing features that are susceptible to diabetic conditions, photoreceptor cells have received relatively little attention with respect to the development of DR. Until recently, studies have suggested that photoreceptors secret proinflammatory molecules and produce reactive oxygen species that contribute to the development of DR. However, the effect of hyperglycemia on photoreceptors and its underlying mechanism remains elusive. In this study, the direct effect of high glucose on photoreceptor cells was investigated using a 661w photoreceptor-like cell line. A data-independent sequential window acquisition of all theoretical mass spectra (SWATH)-based proteomic approach was employed to study changes induced by high glucose in the proteomic profile of the cells. The results indicated that high glucose induced a significant increase in apoptosis and ROS levels in the 661w cells, with mitochondrial dysfunction among the major affected canonical pathways. The involvement of mitochondrial dysfunction was further supported by increased mitochondrial fission and reduced mitochondrial bioenergetics. Collectively, these findings provide a biological basis for a possible role of photoreceptors in the pathogenesis of DR. | en_US |
dcterms.abstract | The following publication Lam, C.H.-I.; Cheung, J.K.-W.;Tse, D.Y.-Y.; Lam, T.C. Proteomic Profiling Re-vealed Mitochondrial Dysfunction in Photoreceptor Cells under Hyperglycemia. Int. J. Mol. Sci. 2022, 23(21), 13366. https://doi.org/10.3390/ijms232113366 | en_US |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | International journal of molecular sciences, 1 Nov. 2022, v. 23, no. 21, 13366 | en_US |
dcterms.isPartOf | International journal of molecular sciences | en_US |
dcterms.issued | 2022-11-01 | - |
dc.identifier.eissn | 1422-0067 | en_US |
dc.identifier.artn | 13366 | en_US |
dc.description.validate | 202211 bcch | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | a1810-n01, a2329 | - |
dc.identifier.SubFormID | 47518 | - |
dc.description.fundingSource | RGC | en_US |
dc.description.fundingSource | Others | en_US |
dc.description.fundingText | Research Centre for SHARP Vision; InnoHK scheme of the Hong Kong Special Administrative Region Government | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Lam_Proteomic_Mitochondrial_Photoreceptor.pdf | 1.55 MB | Adobe PDF | View/Open |
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