Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/95442
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | en_US |
dc.contributor | Research Institute for Future Food | - |
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Huang, Y | en_US |
dc.creator | Yin, H | en_US |
dc.creator | Li, B | en_US |
dc.creator | Wu, Q | en_US |
dc.creator | Liu, Y | en_US |
dc.creator | Poljak, K | en_US |
dc.creator | Maldutyte, J | en_US |
dc.creator | Tang, X | en_US |
dc.creator | Wang, M | en_US |
dc.creator | Wu, Z | en_US |
dc.creator | Miller, EA | en_US |
dc.creator | Jiang, L | en_US |
dc.creator | Yao, ZP | en_US |
dc.creator | Guo, Y | en_US |
dc.date.accessioned | 2022-09-19T02:00:56Z | - |
dc.date.available | 2022-09-19T02:00:56Z | - |
dc.identifier.issn | 0027-8424 | en_US |
dc.identifier.uri | http://hdl.handle.net/10397/95442 | - |
dc.language.iso | en | en_US |
dc.publisher | National Academy of Sciences | en_US |
dc.rights | Copyright © 2021 the Author(s). Published by PNAS. | en_US |
dc.rights | This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/). | en_US |
dc.rights | The following publication Huang, Y., Yin, H., Li, B., Wu, Q., Liu, Y., Poljak, K., ... & Guo, Y. (2021). An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking. Proceedings of the National Academy of Sciences, 118(35), e2101287118 is available at https://doi.org/10.1073/pnas.2101287118 | en_US |
dc.subject | Cargo receptor | en_US |
dc.subject | Cargo sorting | en_US |
dc.subject | COPII | en_US |
dc.subject | Intracellular protein transport | en_US |
dc.subject | Secretory pathway | en_US |
dc.title | An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.volume | 118 | en_US |
dc.identifier.issue | 35 | en_US |
dc.identifier.doi | 10.1073/pnas.2101287118 | en_US |
dcterms.abstract | The fidelity of protein transport in the secretory pathway relies on the accurate sorting of proteins to their correct destinations. To deepen our understanding of the underlying molecular mechanisms, it is important to develop a robust approach to systematically reveal cargo proteins that depend on specific sorting machinery to be enriched into transport vesicles. Here, we used an in vitro assay that reconstitutes packaging of human cargo proteins into vesicles to quantify cargo capture. Quantitative mass spectrometry (MS) analyses of the isolated vesicles revealed cytosolic proteins that are associated with vesicle membranes in a GTP-dependent manner. We found that two of them, FAM84B (also known as LRAT domain containing 2 or LRATD2) and PRRC1, contain proline-rich domains and regulate anterograde trafficking. Further analyses revealed that PRRC1 is recruited to endoplasmic reticulum (ER) exit sites, interacts with the inner COPII coat, and its absence increases membrane association of COPII. In addition, we uncovered cargo proteins that depend on GTP hydrolysis to be captured into vesicles. Comparing control cells with cells depleted of the cargo receptors, SURF4 or ERGIC53, we revealed specific clients of each of these two export adaptors. Our results indicate that the vesicle formation assay in combination with quantitative MS analysis is a robust and powerful tool to uncover novel factors that mediate vesicular trafficking and to uncover cargo clients of specific cellular factors. | en_US |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Proceedings of the National Academy of Sciences of the United States of America, 31 Aug. 2021, v. 118, no. 35, e2101287118 | en_US |
dcterms.isPartOf | Proceedings of the National Academy of Sciences of the United States of America | en_US |
dcterms.issued | 2021-08-31 | - |
dc.identifier.scopus | 2-s2.0-85113481544 | - |
dc.identifier.pmid | 34433667 | - |
dc.identifier.ros | 2021004349 | - |
dc.identifier.eissn | 1091-6490 | en_US |
dc.identifier.artn | e2101287118 | en_US |
dc.description.validate | 202209 bchy | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | CDCF_2021-2022 | - |
dc.description.fundingSource | RGC | en_US |
dc.description.fundingSource | Others | en_US |
dc.description.fundingText | National Natural Science Foundation of China; Shenzhen Science and Technology Innovation Committee; Innovation and Technology Commission; Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou); Medical Research Council; University Research Facility in Chemical and Environmental Analysis and University Research Facility in Life Sciences of PolyU | en_US |
dc.description.pubStatus | Published | en_US |
dc.identifier.OPUS | 71004575 | - |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Huang_vitro_vesicle_formation.pdf | 2.03 MB | Adobe PDF | View/Open |
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