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http://hdl.handle.net/10397/94422
Title: | Exploiting the potential of commercial objectives to extend the field of view of two-photon microscopy by adaptive optics | Authors: | Yao, J Gao, Y Yin, Y Lai, P Ye, S Zheng, W |
Issue Date: | 15-Feb-2022 | Source: | Optics letters, 15 Feb. 2022, v. 47, no. 4, p. 989-992 | Abstract: | Two-photon microscopy (TPM) has provided critical in situ and in vivo information in biomedical studies due to its high resolution, intrinsic optical sectioning, and deep penetration. However, its relatively small field of view (FOV), which is usually determined by objectives, restricts its wide application. In this paper, we propose a segment-scanning sensorless adaptive optics method to extend the FOV and achieve high-resolution and large-FOV two-photon imaging. We demonstrated the proposed method by imaging fluorescent beads, cerebral nerve cells of mouse brain slices, and cerebral vasculature and microglia of live mice. The method extended the FOV of a commercial objective from 1.8 to 3.46 mm while maintaining a lateral resolution of 840 nm and high signal-to-noise ratio. Our technology is compatible with a standard TPM and can be used for large-scale biological exploration. | Publisher: | Optical Society of America | Journal: | Optics letters | ISSN: | 0146-9592 | EISSN: | 1539-4794 | DOI: | 10.1364/OL.450973 | Rights: | © 2022 Optica Publishing Group © 2022 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited. The following publication Jing Yao, Yufeng Gao, Yixuan Yin, Puxiang Lai, Shiwei Ye, and Wei Zheng, "Exploiting the potential of commercial objectives to extend the field of view of two-photon microscopy by adaptive optics," Opt. Lett. 47, 989-992 (2022) is available at https://dx.doi.org/10.1364/OL.450973. |
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