Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/94319
DC Field | Value | Language |
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dc.contributor | Department of Applied Biology and Chemical Technology | en_US |
dc.contributor | Research Institute for Future Food | en_US |
dc.creator | Chan, KKY | en_US |
dc.creator | Kong, HK | en_US |
dc.creator | Tse, SPK | en_US |
dc.creator | Chan, Z | en_US |
dc.creator | Lo, PY | en_US |
dc.creator | Kwok, KWH | en_US |
dc.creator | Lo, SCL | en_US |
dc.date.accessioned | 2022-08-11T02:01:52Z | - |
dc.date.available | 2022-08-11T02:01:52Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/94319 | - |
dc.language.iso | en | en_US |
dc.publisher | Molecular Diversity Preservation International (MDPI) | en_US |
dc.rights | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | en_US |
dc.rights | The following publication Chan, K. K. Y., Kong, H. K., Tse, S. P. K., Chan, Z., Lo, P. Y., Kwok, K. W. H., & Lo, S. C. L. (2021). Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates. Toxins, 13(9), 624 is available at https://doi.org/10.3390/toxins13090624 | en_US |
dc.subject | Cell surface labeling | en_US |
dc.subject | Orthogroup inference | en_US |
dc.subject | Proteomics | en_US |
dc.subject | Toxic dinoflagellates | en_US |
dc.title | Finding species-specific extracellular surface-facing proteomes in toxic dinoflagellates | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.volume | 13 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.doi | 10.3390/toxins13090624 | en_US |
dcterms.abstract | As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAPE) to label and capture species-specific surface proteins (SSSPs) as well as saxitoxins-producing-species-specific surface proteins (Stx-SSPs) that face the extracellular space (i.e., SSSPsEf and Stx-SSPsEf ). Five selected toxic dinoflagellates, Alexandrium minutum, A. lusitanicum, A. tamarense, Gymnodinium catenatum, and Karenia mikimotoi, were used in this study. Transcrip-tomic databases of these five species were also constructed. With the aid of liquid chromatography linked-tandem mass spectrometry (LC-MS/MS) and the transcriptomic databases of these species, extracellularly facing membrane proteomes of the five different species were identified. Within these proteomes, 16 extracellular-facing and functionally significant transport proteins were found. Fur-thermore, 10 SSSPs and 6 Stx-SSPs were identified as amphiesmal proteins but not facing outward to the extracellular environment. We also found SSSPsEf and Stx-SSPsEf in the proteomes. The potential functional correlation of these proteins towards the production of saxitoxins in dinoflagellates and the degree of species specificity were discussed accordingly. | en_US |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Toxins, Sept. 2021, v. 13, no. 9, 624 | en_US |
dcterms.isPartOf | Toxins | en_US |
dcterms.issued | 2021-09 | - |
dc.identifier.scopus | 2-s2.0-85114624019 | - |
dc.identifier.pmid | 34564629 | - |
dc.identifier.eissn | 2072-6651 | en_US |
dc.identifier.artn | 624 | en_US |
dc.description.validate | 202207 bckw | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | a1487,a2225 | - |
dc.identifier.SubFormID | 45131, 47114 | - |
dc.description.fundingSource | RGC | en_US |
dc.description.fundingSource | Others | en_US |
dc.description.fundingText | Environment and Conservation Fund | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
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toxins-13-00624-v2.pdf | 1 MB | Adobe PDF | View/Open |
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