Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/94146
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Biomedical Engineering | en_US |
dc.creator | Liang, Y | en_US |
dc.creator | Xu, X | en_US |
dc.creator | Xu, L | en_US |
dc.creator | Iqbal, Z | en_US |
dc.creator | Ouyang, K | en_US |
dc.creator | Zhang, H | en_US |
dc.creator | Wen, C | en_US |
dc.creator | Duan, L | en_US |
dc.creator | Xia, J | en_US |
dc.date.accessioned | 2022-08-11T01:07:25Z | - |
dc.date.available | 2022-08-11T01:07:25Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/94146 | - |
dc.language.iso | en | en_US |
dc.publisher | Ivyspring International Publisher | en_US |
dc.rights | © The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. | en_US |
dc.rights | The following publication Liang Y, Xu X, Xu L, Iqbal Z, Ouyang K, Zhang H, Wen C, Duan L, Xia J. Chondrocyte-specific genomic editing enabled by hybrid exosomes for osteoarthritis treatment. Theranostics 2022; 12(11):4866-4878 is available at https://dx.doi.org/10.7150/thno.69368. | en_US |
dc.subject | Cartilage | en_US |
dc.subject | CRISPR/Cas9 | en_US |
dc.subject | Hybrid exosome | en_US |
dc.subject | MMP-13 | en_US |
dc.subject | Osteoarthritis | en_US |
dc.subject | Therapeutic genome editing | en_US |
dc.title | Chondrocyte-specific genomic editing enabled by hybrid exosomes for osteoarthritis treatment | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 4866 | en_US |
dc.identifier.epage | 4878 | en_US |
dc.identifier.volume | 12 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.doi | 10.7150/thno.69368 | en_US |
dcterms.abstract | Rationale: A cell-specific delivery vehicle is required to achieve gene editing of the disease-associated cells, so the hereditable genome editing reactions are confined within these cells without affecting healthy cells. A hybrid exosome-based nano-sized delivery vehicle derived by fusion of engineered exosomes and liposomes will be able to encapsulate and deliver CRISPR/Cas9 plasmids selectively to chondrocytes embedded in articular cartilage and attenuate the condition of cartilage damage. | en_US |
dcterms.abstract | Methods: Chondrocyte-targeting exosomes (CAP-Exo) were constructed by genetically fusing a chondrocyte affinity peptide (CAP) at the N-terminus of the exosomal surface protein Lamp2b. Membrane fusion of the CAP-Exo with liposomes formed hybrid CAP-exosomes (hybrid CAP-Exo) which were used to encapsulate CRISPR/Cas9 plasmids. By intra-articular (IA) administration, hybrid CAP-Exo/Cas9 sgMMP-13 entered the chondrocytes of rats with cartilage damages that mimicked the condition of osteoarthritis. | en_US |
dcterms.abstract | Results: The hybrid CAP-Exo entered the deep region of the cartilage matrix in arthritic rats on IA administration, delivered the plasmid Cas9 sgMMP-13 to chondrocytes, knocked down the matrix metalloproteinase 13 (MMP-13), efficiently ablated the expression of MMP-13 in chondrocytes, and attenuated the hydrolytic degradation of the extracellular matrix proteins in the cartilage. | en_US |
dcterms.abstract | Conclusion: Chondrocyte-specific knockdown of MMP-13 mitigates or prevents cartilage degradation in arthritic rats, showing that hybrid CAP-Exo/Cas9 sgMMP-13 may alleviate osteoarthritis. | en_US |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Theranostics, 2022, v. 12, no. 11, p. 4866-4878 | en_US |
dcterms.isPartOf | Theranostics | en_US |
dcterms.issued | 2022 | - |
dc.identifier.scopus | 2-s2.0-85133768458 | - |
dc.identifier.eissn | 1838-7640 | en_US |
dc.description.validate | 202208 bcrc | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | a1622 | - |
dc.identifier.SubFormID | 45635 | - |
dc.description.fundingSource | Self-funded | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
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File | Description | Size | Format | |
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v12p4866.pdf | 2.98 MB | Adobe PDF | View/Open |
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