Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/93820
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | en_US |
dc.creator | Cheung, CY | en_US |
dc.creator | Huang, TT | en_US |
dc.creator | Chow, N | en_US |
dc.creator | Zhang, S | en_US |
dc.creator | Zhao, Y | en_US |
dc.creator | Chau, MP | en_US |
dc.creator | Chan, WC | en_US |
dc.creator | Wong, CCL | en_US |
dc.creator | Boassa, D | en_US |
dc.creator | Phan, S | en_US |
dc.creator | Ellisman, MH | en_US |
dc.creator | Yates, JR | en_US |
dc.creator | Xu, S | en_US |
dc.creator | Yu, Z | en_US |
dc.creator | Zhang, Y | en_US |
dc.creator | Zhang, R | en_US |
dc.creator | Ng, LL | en_US |
dc.creator | Ko, BCB | en_US |
dc.date.accessioned | 2022-08-01T06:00:18Z | - |
dc.date.available | 2022-08-01T06:00:18Z | - |
dc.identifier.issn | 0021-9533 | en_US |
dc.identifier.uri | http://hdl.handle.net/10397/93820 | - |
dc.language.iso | en | en_US |
dc.publisher | Company of Biologists | en_US |
dc.rights | © 2022. Published by The Company of Biologists Ltd | en_US |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. | en_US |
dc.rights | The following publication Chris Y. Cheung, Ting-Ting Huang, Ning Chow, Shuqi Zhang, Yanxiang Zhao, Mary P. Chau, Wing Cheung Chan, Catherine C. L. Wong, Daniela Boassa, Sebastien Phan, Mark H. Ellisman, John R. Yates, SongXiao Xu, Zicheng Yu, Yajing Zhang, Rui Zhang, Ling Ling Ng, Ben C. B. Ko; Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2. J Cell Sci 1 July 2022; 135 (13): jcs259280 is available at https://doi.org/10.1242/jcs.259280. | en_US |
dc.subject | Exportin-T | en_US |
dc.subject | Importin | en_US |
dc.subject | NFAT5 | en_US |
dc.subject | Nucleocytoplasmic trafficking | en_US |
dc.subject | RUVBL2 | en_US |
dc.title | Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2 | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.volume | 135 | en_US |
dc.identifier.issue | 13 | en_US |
dc.identifier.doi | 10.1242/jcs.259280 | en_US |
dcterms.abstract | NFAT5 is the only known mammalian tonicity-responsive transcription factor with an essential role in cellular adaptation to hypertonic stress. It is also implicated in diverse physiological and pathological processes. NFAT5 activity is tightly regulated by extracellular tonicity, but the underlying mechanisms remain elusive. Here, we demonstrate that NFAT5 enters the nucleus via the nuclear pore complex. We found that NFAT5 utilizes a unique nuclear localization signal (NFAT5-NLS) for nuclear import. siRNA screening revealed that only karyopherin β1 (KPNB1), but not karyopherin α, is responsible for the nuclear import of NFAT5 via direct interaction with the NFAT5-NLS. Proteomics analysis and siRNA screening further revealed that nuclear export of NFAT5 under hypotonicity is driven by exportin-T (XPOT), where the process requires RuvB-like AAA-type ATPase 2 (RUVBL2) as an indispensable chaperone. Our findings have identified an unconventional tonicity-dependent nucleocytoplasmic trafficking pathway for NFAT5 that represents a critical step in orchestrating rapid cellular adaptation to change in extracellular tonicity. These findings offer an opportunity for the development of novel NFAT5 targeting strategies that are potentially useful for the treatment of diseases associated with NFAT5 dysregulation. | en_US |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Journal of cell science, July 2022, v. 135, no. 13, jcs259280 | en_US |
dcterms.isPartOf | Journal of cell science | en_US |
dcterms.issued | 2022-07 | - |
dc.identifier.scopus | 2-s2.0-85134426501 | - |
dc.identifier.eissn | 1477-9137 | en_US |
dc.identifier.artn | jcs259280 | en_US |
dc.description.validate | 202208_bcww | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_TA | - |
dc.description.fundingSource | RGC | en_US |
dc.description.fundingSource | Others | en_US |
dc.description.fundingText | Hong Kong Polytechnic University; National Institutes of Health; National Institutes of Health | en_US |
dc.description.pubStatus | Published | en_US |
dc.description.TA | CompOfBiologists (2022) | en_US |
dc.description.oaCategory | TA | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cheung_Unconventional_Tonicity-regulated_Nuclear.pdf | 15.5 MB | Adobe PDF | View/Open |
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