Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/93493
DC Field | Value | Language |
---|---|---|
dc.contributor | School of Optometry | en_US |
dc.contributor | School of Optometry | - |
dc.contributor | Research Centre for SHARP Vision | - |
dc.contributor | Research Centre for Chinese Medicine Innovation | - |
dc.creator | Li, H | en_US |
dc.creator | Shan, SW | en_US |
dc.creator | Stamer, WD | en_US |
dc.creator | Li, K | en_US |
dc.creator | Chan, HH | en_US |
dc.creator | Civan, MM | en_US |
dc.creator | To, C | en_US |
dc.creator | Lam, TC | en_US |
dc.creator | Do, C | en_US |
dc.date.accessioned | 2022-07-05T08:34:48Z | - |
dc.date.available | 2022-07-05T08:34:48Z | - |
dc.identifier.issn | 1661-6596 | en_US |
dc.identifier.uri | http://hdl.handle.net/10397/93493 | - |
dc.language.iso | en | en_US |
dc.publisher | Molecular Diversity Preservation International (MDPI) | en_US |
dc.rights | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | en_US |
dc.rights | The following publication Li, H. L., Shan, S. W., Stamer, W. D., Li, K. K., Chan, H. H. L., Civan, M. M., ... & Do, C. W. (2022). Mechanistic Effects of Baicalein on Aqueous Humor Drainage and Intraocular Pressure. International Journal of Molecular Sciences, 23(13), 7372 is available at https://doi.org/10.3390/ijms23137372 | en_US |
dc.subject | Baicalein | en_US |
dc.subject | Intraocular pressure | en_US |
dc.subject | Outflow facility | en_US |
dc.subject | Trabecular meshwork | en_US |
dc.subject | Cell contractility | en_US |
dc.subject | Regulatory volume decrease | en_US |
dc.subject | Extracellular matrix | en_US |
dc.subject | Proteomics | en_US |
dc.subject | Glaucoma | en_US |
dc.title | Mechanistic effects of Baicalein on aqueous humor drainage and intraocular pressure | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.volume | 23 | en_US |
dc.identifier.issue | 13 | en_US |
dc.identifier.doi | 10.3390/ijms23137372 | en_US |
dcterms.abstract | Elevated intraocular pressure (IOP) is a major risk factor for glaucoma that results from impeded fluid drainage. The increase in outflow resistance is caused by trabecular meshwork (TM) cell dysfunction and excessive extracellular matrix (ECM) deposition. Baicalein (Ba) is a natural flavonoid and has been shown to regulate cell contraction, fluid secretion, and ECM remodeling in various cell types, suggesting the potential significance of regulating outflow resistance and IOP. We demonstrated that Ba significantly lowered the IOP by about 5 mmHg in living mice. Consistent with that, Ba increased the outflow facility by up to 90% in enucleated mouse eyes. The effects of Ba on cell volume regulation and contractility were examined in primary human TM (hTM) cells. We found that Ba (1–100 µM) had no effect on cell volume under iso-osmotic conditions but inhibited the regulatory volume decrease (RVD) by up to 70% under hypotonic challenge. In addition, Ba relaxed hTM cells via reduced myosin light chain (MLC) phosphorylation. Using iTRAQ-based quantitative proteomics, 47 proteins were significantly regulated in hTM cells after a 3-h Ba treatment. Ba significantly increased the expression of cathepsin B by 1.51-fold and downregulated the expression of D-dopachrome decarboxylase and pre-B-cell leukemia transcription factor-interacting protein 1 with a fold-change of 0.58 and 0.40, respectively. We suggest that a Ba-mediated increase in outflow facility is triggered by cell relaxation via MLC phosphorylation along with inhibiting RVD in hTM cells. The Ba-mediated changes in protein expression support the notion of altered ECM homeostasis, potentially contributing to a reduction of outflow resistance and thereby IOP. | en_US |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | International journal of molecular sciences, July 2022, v. 23, no. 13, 7372 | en_US |
dcterms.isPartOf | International journal of molecular sciences | en_US |
dcterms.issued | 2022-07 | - |
dc.identifier.eissn | 1422-0067 | en_US |
dc.identifier.artn | 7372 | en_US |
dc.description.validate | 202207 bckw | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | a1541-n01, a2183 | - |
dc.identifier.SubFormID | 46921 | - |
dc.description.fundingSource | Others | en_US |
dc.description.fundingText | Health Medical Research Fund; PolyU grants; The Government of the Hong Kong Special Administrative Region & Innovation and Technology Fund; Shenzhen Science and Technology Innovation Commission; Henry G. Leong Endowed Professorship in Elderly Vision Health; National Eye Institute, USA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
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ijms-23-07372.pdf | 1.27 MB | Adobe PDF | View/Open |
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