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http://hdl.handle.net/10397/90870
Title: | Worm-based microfluidic biosensor for real-time assessment of the metastatic status | Authors: | Zhang, J Chua, SL Khoo, BL |
Issue Date: | Feb-2021 | Source: | Cancers, Feb. 2021, v. 13, no. 4, 873, p. 1-14 | Abstract: | Background: Metastasis is a complex process that affects patient treatment and survival. To routinely monitor cancer plasticity and guide treatment strategies, it is highly desired to provide information about metastatic status in real-time. Here, we proposed a worm-based (WB) microfluidic biosensor to rapidly monitor biochemical cues related to metastasis in a well-defined environment. Compared to conventional biomarker-based methods, the WB biosensor allowed high throughput screening under low cost, requiring only visual quantification of outputs; Methods: Cae-norhabditis elegans were placed in the WB biosensor and exposed to samples conditioned with cancer cell clusters. The chemotactic preference of these worms was observed under discontinuous imaging to minimize the impact on physiological activity; Results: A chemotaxis index (CI) was defined to standardize the quantitative assessment from the WB biosensor, where moderate (3.24–6.5) and high (>6.5) CI levels reflected increased metastasis risk and presence of metastasis, respectively. We demonstrated that the secreted metabolite glutamate was a chemorepellent, and larger clusters associated with increased metastatic potential also enhanced CI levels; Conclusions: Overall, this study provided a proof of concept for the WB biosensors in assessing metastasis status, with the potential to evaluate patient-derived cancer clusters for routine management. | Keywords: | Disease monitoring Label-free Metastasis Preclinical models Worm-based biosensor |
Publisher: | MDPI AG | Journal: | Cancers | EISSN: | 2072-6694 | DOI: | 10.3390/cancers13040873 | Rights: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). The following publication Zhang, J.; Chua, S.L.; Khoo, B.L.Worm-Based Microfluidic Biosensor for Real-Time Assessment of the Metastatic Status. Cancers 2021, 13, 873 is available at https://doi.org/10.3390/cancers13040873 |
Appears in Collections: | Journal/Magazine Article |
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cancers-13-00873-v2.pdf | 1.89 MB | Adobe PDF | View/Open |
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