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Title: | High prevalence and mechanism associated with extended spectrum beta-lactamase-positive phenotype in Laribacter hongkongensis | Authors: | Teng, JLL Luo, R Tang, BSF Fong, JYH Wang, L Jia, L Wong, CKS Chan, E Leung, AWS Siu, GKH Chiu, TH Fung, AMY Wu, AKL Yeung, ML Lau, SKP Woo, PCY |
Issue Date: | Feb-2021 | Source: | Frontiers in microbiology, Feb. 2021, v. 12, 618894 | Abstract: | In this study, we reported the prevalence and mechanism associated with the extended-spectrum beta-lactamase (ESBL)-positive phenotype in Laribacter hongkongensis isolated from patients and fish. Using the inhibition zone enhancement test, 20 (95.2%) of the 21 patient strains and 8 (57.1%) of the 14 fish strains were tested ESBL-positive. However, ESBL genes, including SHV, TEM, CTX-M, GES, and PER, were not detected in all of these 28 L. hongkongensis isolates. No ESBL gene could be detected in either the complete genome of L. hongkongensis HLHK9 or the draft genome of PW3643. PCR and DNA sequencing revealed that all the 35 L. hongkongensis isolates (showing both ESBL-positive and ESBL-negative phenotypes) were positive for the ampC gene. When the AmpC deletion mutant, HLHK9ΔampC, was subject to the zone enhancement test, the difference of zone size between ceftazidime/clavulanate and ceftazidime was less than 5 mm. When boronic acid was added to the antibiotic disks, none of the 28 “ESBL-positive” isolates showed a ≥ 5 mm enhancement of inhibition zone size diameter between ceftazidime/clavulanate and ceftazidime and between cefotaxime/clavulanate and cefotaxime. A high prevalence (80%) of ESBL-positive phenotype is present in L. hongkongensis. Overall, our results suggested that the ESBL-positive phenotype in L. hongkongensis results from the expression of the intrinsic AmpC beta-lactamase. Confirmatory tests should be performed before issuing laboratory reports for L. hongkongensis isolates that are tested ESBL-positive by disk diffusion clavulanate inhibition test. | Keywords: | AmpC beta-lactamase ESBL Laribacter hongkongensis Mechanism Prevalence |
Publisher: | Frontiers Research Foundation | Journal: | Frontiers in microbiology | ISSN: | 1664-302X | DOI: | 10.3389/fmicb.2021.618894 | Rights: | © 2021 Teng, Luo, Tang, Fong, Wang, Jia, Wong, Chan, Leung, Siu, Chiu, Fung, Wu, Yeung, Lau and Woo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. The following publication Teng JLL, Luo R, Tang BSF, Fong JYH, Wang L, Jia L, Wong CKS, Chan E, Leung AWS, Siu GKH, Chiu T-H, Fung AMY, Wu AKL, Yeung M-L, Lau SKP and Woo PCY (2021) High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis. Front. Microbiol. 12:618894 is available at https://doi.org/10.3389/fmicb.2021.618894 |
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