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http://hdl.handle.net/10397/89886
Title: | Optical-resolution photoacoustic microscopy with ultrafast dual-wavelength excitation | Authors: | Zhou, Y Liang, S Li, M Liu, C Lai, P Wang, L |
Issue Date: | Jun-2020 | Source: | Journal of biophotonics, June 2020, v. 13, no. 6, e201960229, p. 1-7 | Abstract: | Fast functional and molecular photoacoustic microscopy requires pulsed laser excitations at multiple wavelengths with enough pulse energy and short wavelength-switching time. Recent development of stimulated Raman scattering in optical fiber offers a low-cost laser source for multiwavelength photoacoustic imaging. In this approach, long fibers temporally separate different wavelengths via optical delay. The time delay between adjacent wavelengths may eventually limits the highest A-line rate. In addition, a long-time delay in fiber may limit the highest pulse energy, leading to poor image quality. In order to achieve high pulse energy and ultrafast dual-wavelength excitation, we present optical-resolution photoacoustic microscopy with ultrafast dual-wavelength excitation and a signal separation method. The signal separation method is validated in numerical simulation and phantom experiments. We show that when two photoacoustic signals are partially overlapped with a 50-ns delay, they can be recovered with 98% accuracy. We apply this ultrafast dual-wavelength excitation technique to in vivo OR-PAM. Results demonstrate that A-lines at two wavelengths can be successfully separated, and sO2 values can be reliably computed from the separated data. The ultrafast dual-wavelength excitation enables fast functional photoacoustic microscopy with negligible misalignment among different wavelengths and high pulse energy, which is important for in vivo imaging of microvascular dynamics. | Keywords: | Fast dual-wavelength excitation Functional photoacoustic imaging Multiwavelength Signal separation |
Publisher: | Wiley-VCH | Journal: | Journal of biophotonics | ISSN: | 1864-063X | EISSN: | 1864-0648 | DOI: | 10.1002/jbio.201960229 | Rights: | © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim This is the peer reviewed version of the following article: Zhou, Y, Liang, S, Li, M, Liu, C, Lai, P, Wang, L. Optical-resolution photoacoustic microscopy with ultrafast dual-wavelength excitation. J. Biophotonics. 2020; 13:e201960229, which has been published in final form at https://doi.org/10.1002/jbio.201960229. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
Appears in Collections: | Journal/Magazine Article |
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1805_2020_JoB_Yingying.pdf | Pre-Published version | 1.61 MB | Adobe PDF | View/Open |
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