Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/89179
DC Field | Value | Language |
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dc.contributor | Department of Health Technology and Informatics | - |
dc.creator | Shenkutie, AM | - |
dc.creator | Yao, MZ | - |
dc.creator | Siu, GKH | - |
dc.creator | Wong, BKC | - |
dc.creator | Leung, PHM | - |
dc.date.accessioned | 2021-02-04T02:40:02Z | - |
dc.date.available | 2021-02-04T02:40:02Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/89179 | - |
dc.language.iso | en | en_US |
dc.publisher | MDPI AG | en_US |
dc.rights | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | en_US |
dc.rights | The following publication Shenkutie, A. M., Yao, M. Z., Siu, G. K. -., Wong, B. K. C., & Leung, P. H. -. (2020). Biofilm-induced antibiotic resistance in clinical acinetobacter baumannii isolates. Antibiotics, 9(11), 817, 1-15 is available at https://dx.doi.org/10.3390/antibiotics9110817 | en_US |
dc.subject | Acinetobacter baumannii | en_US |
dc.subject | Antibiotic resistance | en_US |
dc.subject | Antibiotic tolerance | en_US |
dc.subject | Biofilm | en_US |
dc.subject | Persister | en_US |
dc.title | Biofilm-induced antibiotic resistance in clinical acinetobacter baumannii isolates | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 15 | - |
dc.identifier.volume | 9 | - |
dc.identifier.issue | 11 | - |
dc.identifier.doi | 10.3390/antibiotics9110817 | - |
dcterms.abstract | In order to understand the role of biofilm in the emergence of antibiotic resistance, a total of 104 clinical Acinetobacter baumannii strains were investigated for their biofilm-forming capacities and genes associated with biofilm formation. Selected biofilm-formers were tested for antibiotic susceptibilities when grown in biofilm phase. Reversibility of antibiotic susceptibility in planktonic cells regrown from biofilm were investigated. We found 59.6% of the strains were biofilm-formers, among which, 66.1% were non-multidrug resistant (MDR) strains. Presence of virulence genes bap, csuE, and abaI was significantly associated with biofilm-forming capacities. When strains were grown in biofilm state, the minimum biofilm eradication concentrations were 44, 407, and 364 times higher than the minimum bactericidal concentrations (MBC) for colistin, ciprofloxacin, and imipenem, respectively. Persisters were detected after treating the biofilm at 32–256 times the MBC of planktonic cells. Reversibility test for antibiotic susceptibility showed that biofilm formation induced reversible antibiotic tolerance in the non-MDR strains but a higher level of irreversible resistance in the extensively drug-resistant (XDR) strain. In summary, we showed that the non-MDR strains were strong biofilm-formers. Presence of persisters in biofilm contributed to the reduced antibiotic susceptibilities. Biofilm-grown Acinetobacter baumannii has induced antibiotic tolerance in non-MDR strains and increased resistance levels in XDR strains. To address the regulatory mechanisms of biofilm-specific resistance, thorough investigations at genome and transcription levels are warranted. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Antibiotics, Nov. 2020, v. 9, no. 11, 817, p. 1-15 | - |
dcterms.isPartOf | Antibiotics | - |
dcterms.issued | 2020-11 | - |
dc.identifier.isi | WOS:000593516400001 | - |
dc.identifier.scopus | 2-s2.0-85096520469 | - |
dc.identifier.eissn | 2079-6382 | - |
dc.identifier.artn | 817 | - |
dc.description.validate | 202101 bcrc | - |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_Scopus/WOS | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
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File | Description | Size | Format | |
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antibiotics-09-00817.pdf | 3.89 MB | Adobe PDF | View/Open |
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