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Title: | Increased intracellular iron and mineralization of cultured hFOB 1.19 cells following hepcidin activation through ferroportin-1 | Authors: | Zhang, P Xu, YJ Zhao, DY Ma, Y Xiao, L Feng, YS Du, BC Qian, ZM Li, K |
Issue Date: | Dec-2010 | Source: | Saudi medical journal, Dec. 2010, , v. 31, no. 12, p. 1303-1308 | Abstract: | Objectives: To address whether hepcidin functions in bone metabolism. Methods: This study was carried out in the Laboratory of Radiation Medicine and Public Health of Soochow University, and the Laboratory of the Second Affiliated Hospital of Soochow University, Suzhou, China, from September 2009 to July 2010. The positive expression of ferroportin-1 (Fpn-1) was detected by reverse transcriptase-polymerase chain reaction. After the treatment with distilled water (control group) and hepcidin (25noml/L, 50noml/L, 100noml/L), the fluorescence intensity related to intracellular iron concentration of a human fetal osteoblast cell line (hFOB 1.19) was measured by a confocal laser scanning microscope. A 3-(4,5- dimethylthiazol-2-y1) - 2-5-diphenyltetrazolium bromide assay, and Von Kossa staining was performed to evaluate cell proliferation and mineralization in cultured hFOB 1.19 cells. Results: This study revealed a high level expression of Fpn-1 in hFOB 1.19. On the basis of which, it was found that 25noml/L, 50noml/L, 100noml/L hepcidin could promote the fluorescence intensity related to intracellular iron concentration and mineralization in hFOB 1.19 in a dose-dependent manner (p<0.05), but hepcidin had no effect on FOB 1.19 proliferation (p>0.05). Conclusion: The hepcidin-ferroportin signal pathway may function in the osteoblast cell line of hFOB 1.19 cells. It is also suggested that cross-talk between iron and calcium homeostasis may play a role in bone metabolism in responding to hepcidin activation. |
Publisher: | Saudi Arabian Armed Forces Ministry of Defence and Aviation, Medical Services Department | Journal: | Saudi medical journal | ISSN: | 0379-5284 | EISSN: | 1658-3175 | Rights: | Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention. Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) (https://creativecommons.org/licenses/by-nc/4.0/). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. The following publication Zhang P, Xu YJ, Zhao DY, Ma Y, Xiao L, Feng YS, Du BC, Qian ZM, Li K. Increased intracellular iron and mineralization of cultured hFOB 1.19 cells following hepcidin activation through ferroportin-1. Saudi Med J. 2010 Dec;31(12):1303-8 is available at https://www.smj.org.sa/index.php/smj/article/view/7076 |
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