Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/88055
Title: | Increased connexin36 phosphorylation in AII amacrine cell coupling of the mouse myopic retina | Authors: | Banerjee, S Wang, Q Zhao, FX Tang, G So, C Tse, D To, CH Feng, Y Zhou, XT Pan, F |
Issue Date: | 1-Jun-2020 | Source: | Frontiers in cellular neuroscience, 1 June 2020, v. 14, 124, p. 1-17 | Abstract: | Myopia is a substantial public health problem worldwide. In the myopic retina, distant images are focused in front of the photoreceptors. The cells and mechanisms for retinal signaling that account either for emmetropization (i.e., normal refraction) or for refractive errors have remained elusive. Gap junctions play a key component in enhancement of signal transmission in visual pathways. AII amacrine cells (ACs), coupled by connexin36, segregate signals into ON and OFF pathways. Coupling between AII ACs is actively modulated through phosphorylation at serine 293 via dopamine in the mouse retina. In this study, form deprivation mouse myopia models were used to evaluate the expression patterns of connexin36-positive plaques (structural assay) and the state of connexin36 phosphorylation (functional assay) in AII ACs, which was green fluorescent protein-expressing in the Fam81a mouse line. Single-cell RNA sequencing showed dopaminergic synapse and gap junction pathways of AII ACs were downregulated in the myopic retina, although Gjd2 mRNA expression remained the same. Compared with the normal refractive eye, phosphorylation of connexin36 was increased in the myopic retina, but expression of connexin36 remained unchanged. This increased phosphorylation of Cx36 could indicate increased functional gap junction coupling of AII ACs in the myopic retina, a possible adaptation to adjust to the altered noisy signaling status. | Keywords: | Amacrine cell Ganglion cell Myopia Retina Gap junction (connexin) |
Publisher: | Frontiers Research Foundation | Journal: | Frontiers in cellular neuroscience | EISSN: | 1662-5102 | DOI: | 10.3389/fncel.2020.00124 | Rights: | © 2020 Banerjee, Wang, Zhao, Tang, So, Tse, To, Feng, Zhou and Pan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. The following publication Banerjee, S., Wang, Q., Zhao, F. X., Tang, G., So, C., Tse, D., . . . Pan, F. (2020). Increased connexin36 phosphorylation in AII amacrine cell coupling of the mouse myopic retina. Frontiers in Cellular Neuroscience, 14, 1-17 is available at https://dx.doi.org/10.3389/fncel.2020.00124 |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Banerjee_Increased_Connexin36_Phosphorylation.pdf | 4.03 MB | Adobe PDF | View/Open |
Page views
138
Last Week
1
1
Last month
Citations as of May 5, 2024
Downloads
110
Citations as of May 5, 2024
SCOPUSTM
Citations
13
Citations as of Apr 4, 2024
WEB OF SCIENCETM
Citations
11
Citations as of May 2, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.