Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/87813
Title: | Brevilin a, a natural sesquiterpene lactone inhibited the growth of triple-negative breast cancer cells Akt/mTOR and STAT3 signaling pathways | Authors: | Qu, Z Lin, YS Mok, DKW Bian, QY Tai, WCS Chen, SB |
Issue Date: | 10-Jun-2020 | Source: | Oncotargets and therapy, 10 June 2020, v. 13, p. 5363-5373 | Abstract: | Purpose: Triple-negative breast cancer (TNBC) is a a breast cancer subtype characterized by a lack of estrogen receptor, progesterone receptor and human epidermal growth receptor 2 and is associated with poorer prognoses when compared to other breast cancers. Thus, novel anti-cancer agents with high efficacy are urgently needed. Brevilin A (BA), a natural sesquiterpene lactone, has been reported to exhibit anti-cancer effects. However, the effects of BA on TNBC have not yet been demonstrated. In this study, we investigated the anti-TNBC effects and the underlying mechanism of BA, in vitro and in vivo. Methods: Two TNBC cell lines and a xenograft mouse model were employed to assess the effects of BA. Cell viability was detected by MTT assay. Cell cycle status and apoptosis were evaluated by flow cytometry. Cell migration was measured by wound-healing assay. Protein expression was measured by Western blotting analysis. The in vivo anti-cancer activity of BA was assessed in orthotopic tumor xenograft mice. Results: BA significantly inhibited the growth of TNBC cells in a dose- and time-dependent manner via induction of cell cycle arrest at G2/M phase arrest and apoptosis. BA also inhibited tumor cell migration. BA significantly downregulated the expression of Akt, mTOR, Stat3 and their phosphorylation, and thus inhibiting the activation of the Akt/mTOR and STAT3 signaling pathways. Furthermore, oral administration of BA at 25 or 50 mg/kg leads to significant inhibition of tumor growth and proliferation in tumor xenograft model mice. Conclusion: BA significantly inhibited the growth and migration of TNBC cells, and induced cell cycle arrest and apoptosis. These inhibitory effects were associated with the suppression of the Akt/mTOR and Stat3 signal pathways. Based on our findings, BA possesses a promising candidate for development as an anti-cancer therapeutic drug against TNBC. |
Keywords: | Anticancer Apoptosis Cell cycle arrest Migration Cancer therapy |
Publisher: | Dove Medical Press | Journal: | Oncotargets and therapy | EISSN: | 1178-6930 | DOI: | 10.2147/OTT.S256833 | Rights: | © 2020 Qu et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). The following publication Qu Z, Lin Y, Mok DKW, Bian Q, Tai WCS, Chen S. Brevilin A, a Natural Sesquiterpene Lactone Inhibited the Growth of Triple-Negative Breast Cancer Cells via Akt/mTOR and STAT3 Signaling Pathways. Onco Targets Ther. 2020;13:5363-5373 is available at https://dx.doi.org/10.2147/OTT.S256833 |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Qu_Brevilin_Sesquiterpene_Lactone.pdf | 13.98 MB | Adobe PDF | View/Open |
Page views
76
Last Week
0
0
Last month
Citations as of Apr 28, 2024
Downloads
31
Citations as of Apr 28, 2024
SCOPUSTM
Citations
13
Citations as of Apr 26, 2024
WEB OF SCIENCETM
Citations
12
Citations as of Apr 25, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.