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http://hdl.handle.net/10397/87761
Title: | A modified arginine-depleting enzyme NEI-01 inhibits growth of pancreatic cancer cells | Authors: | Chow, JPH Cai, YJ Chow, DTL Chung, SHK Chau, KC Ng, KY Leung, OM Wong, RMH Law, AWL Leung, YO Kwok, SY Leung, YC |
Issue Date: | 2020 | Source: | PLoS one, 2020, v. 15, no. 4, e0231633, p. 1-19 | Abstract: | Arginine deprivation cancer therapy targets certain types of malignancies with positive result in many studies and clinical trials. NEI-01 was designed as a novel arginine-depleting enzyme comprising an albumin binding domain capable of binding to human serum albumin to lengthen its half-life. In the present work, NEI-01 is shown to bind to serum albumin from various species, including mice, rat and human. Single intraperitoneal administration of NEI-01 to mice reduced plasma arginine to undetectable level for at least 9 days. Treatment of NEI-01 specifically inhibited cell viability of MIA PaCa-2 and PANC-1 cancer cell lines, which were ASS1 negative. Using a human pancreatic mouse xenograft model, NEI-01 treatment significantly reduced tumor volume and weight. Our data provides proof of principle for a cancer treatment strategy using NEI-01. | Publisher: | Public Library of Science | Journal: | PLoS one | EISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0231633 | Rights: | © 2020 Chow et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The following publication Chow JPH, Cai Y, Chow DTL, Chung SHK, Chau K-C, Ng K-Y, et al. (2020) A modified arginine-depleting enzyme NEI-01 inhibits growth of pancreatic cancer cells. PLoS ONE 15(4): e0231633. is available at https://dx.doi.org/10.1371/journal.pone.0231633 |
Appears in Collections: | Journal/Magazine Article |
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