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Title: | Hrps is a global regulator on type III secretion system (T3SS) and non-T3SS genes in pseudomonas savastanoi pv. phaseolicola | Authors: | Wang, J Shao, X Zhang, Y Zhu, Y Yang, P Yuan, J Wang, T Yin, C Wang, W Chen, S Liang, Haihua Deng, Xin |
Issue Date: | 2018 | Source: | Molecular plant-microbe interactions, Dec. 2018, v. 31, no. 12, p. 1232-1243 | Abstract: | The type III secretion system (T3SS) is the main machinery for Pseudomonas savastanoi and other gram-negative bacteria to invade plant cells. HrpR and HrpS form a hetero-hexamer, which activates the expression of HrpL, which induces all T3SS genes by binding to a ‘hrp box’ in promoters. However, the individual molecular mechanism of HrpR or HrpS has not been fully understood. Through chromatin immunoprecipitation coupled to high-throughput DNA sequencing, we found that HrpR, HrpS, and HrpL had four, 47, and 31 targets on the genome, respectively. HrpS directly bound to the promoter regions of a group of T3SS genes and non-T3SS genes. HrpS independently regulated these genes in a hrpL deletion strain. Additionally, a HrpS-binding motif (GTGCCAAA) was identified, which was verified by electrophoretic mobility shift assay and lux-reporter assay. HrpS also regulated motility and biofilm formation in P. savastanoi. The present study strongly suggests that HrpS alone can work as a global regulator on both T3SS and non-T3SS genes in P. savastanoi. | Publisher: | American Phytopathological Society | Journal: | Molecular plant-microbe interactions | ISSN: | 0894-0282 | EISSN: | 1943-7706 | DOI: | 10.1094/MPMI-02-18-0035-R | Rights: | Copyright © 2018 The Author(s). This is an open-access article distributed under the CC BY-NC-ND 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/). The following publication Wang, J., Shao, X., Zhang, Y., Zhu, Y., Yang, P., Yuan, J., . . . Deng, X. I. N. (2018). Hrps is a global regulator on type III secretion system(T3SS) and non-T3SS genes in pseudomonas savastanoi pv. phaseolicola. Molecular Plant-Microbe Interactions, 31(12), 1232-1243 is available at https://dx.doi.org/10.1094/MPMI-02-18-0035-R |
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