Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/80446
Title: Long non-coding RNAs in hematological malignancies : translating basic techniques into diagnostic and therapeutic strategies
Authors: Wong, NK 
Huang, CL 
Islam, R 
Yip, SP 
Keywords: LncRNAs
Hematological malignancies
Experimental approaches
Genetic manipulation
Biomarkers
Issue Date: 2018
Publisher: BioMed Central
Source: Journal of hematology & oncology, 22 Nov. 2018, v. 11, 131, p. 1-22 How to cite?
Journal: Journal of hematology & oncology 
Abstract: Recent studies have revealed that non-coding regions comprise the vast majority of the human genome and long non-coding RNAs (lncRNAs) are a diverse class of non-coding RNAs that has been implicated in a variety of biological processes. Abnormal expression of lncRNAs has also been linked to different human diseases including cancers, yet the regulatory mechanisms and functional effects of lncRNAs are still ambiguous, and the molecular details also need to be confirmed. Unlike protein-coding gene, it is much more challenging to unravel the roles of lncRNAs owing to their unique and complex features such as functional diversity and low conservation among species, which greatly hamper their experimental characterization. In this review, we summarize and discuss both conventional and advanced approaches for the identification and functional characterization of lncRNAs related to hematological malignancies. In particular, the utility and advancement of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system as gene-editing tools are envisioned to facilitate the molecular dissection of lncRNAs via different knock-in/out strategies. Besides experimental considerations specific to lncRNAs, the roles of lncRNAs in the pathogenesis and progression of leukemia are also highlighted in the review. We expect that these insights may ultimately lead to clinical applications including development of biomarkers and novel therapeutic approaches targeting lncRNAs.
URI: http://hdl.handle.net/10397/80446
EISSN: 1756-8722
DOI: 10.1186/s13045-018-0673-6
Rights: © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
The following publication Wong, N. K., Huang, C. L., Islam, R., & Yip, S. P. (2018). Long non-coding RNAs in hematological malignancies: translating basic techniques into diagnostic and therapeutic strategies. Journal of Hematology & Oncology, 11, 131, 1-22 is available at https://dx.doi.org/10.1186/s13045-018-0673-6
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