Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/80276
Title: Magnetic force-driven in situ selective intracellular delivery
Authors: Wang, R
Chow, YT
Chen, SX
Ma, DC
Luo, T
Tan, YH 
Sun, D
Issue Date: 2018
Publisher: Nature Publishing Group
Source: Scientific reports, Sept. 2018, v. 8, 14205, p. 1-10 How to cite?
Journal: Scientific reportsonline only 
Abstract: Intracellular delivery of functional materials holds great promise in biologic research and therapeutic applications but poses challenges to existing techniques, including the reliance on exogenous vectors and lack of selectivity. To address these problems, we propose a vector-free approach that utilizes millimeter-sized iron rods or spheres driven by magnetic forces to selectively deform targeted cells, which in turn generates transient disruption in cell membranes and enables the delivery of foreign materials into cytosols. A range of functional materials with the size from a few nanometers to hundreds of nanometers have been successfully delivered into various types of mammalian cells in situ with high efficiency and viability and minimal undesired effects. Mechanistically, material delivery is mediated by force-induced transient membrane disruption and restoration, which depend on actin cytoskeleton and calcium signaling. When used for siRNA delivery, CXCR4 is effectively silenced and cell migration and proliferation are significantly inhibited. Remarkably, cell patterns with various complexities are generated, demonstrating the unique ability of our approach in selectively delivering materials into targeted cells in situ. In summary, we have developed a magnetic force-driven intracellular delivery method with in situ selectivity, which may have tremendous applications in biology and medicine.
URI: http://hdl.handle.net/10397/80276
EISSN: 2045-2322
DOI: 10.1038/s41598-018-32605-w
Rights: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2018
The following publication Wang, R., Chow, Y.T., Chen, S.X., Ma, D.C., Luo, T., Tan, Y.H., & Sun, D. (2018). Magnetic force-driven in situ selective intracellular delivery. Scientific reports, 8, 14205, 1-10 is available at https://dx.doi.org/10.1038/s41598-018-32605-w
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