Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/79920
Title: A selective medium for pyocyanin-dependent fast electrochemical detection of pseudomonas aeruginosa in environmental microbial samples
Authors: Li, SH 
Mou, QQ 
Feng, NZ 
Leung, PHM 
Keywords: Pseudomonas aeruginosa
Pyocyanin
Electrochemical detection
Selective medium
Differential pulse voltammetry
Issue Date: 2018
Publisher: Electrochemical Science Group
Source: International journal of electrochemical science, Apr. 2018, v. 13, no. 4, p. 3789-3798 How to cite?
Journal: International journal of electrochemical science 
Abstract: In this work, a selective medium was developed for pyocyanina-dependent qualitative detection of Pseudomonas aeruginosa in raw microbial samples using electrochemical analysis. To obtain an optimum nutritive formulation, the effect of different media (BYEB, LB, SDB, MHB, TSB, and NB), positive ions (K+, Na+, Ca2+, Fe3+, and Mg2+) and selected additives (1-naphthylamine, acetamide, and benzylpenicillin sodium) on the amount of synthesized pyocyanin were investigated and compared. NB was found to be the most favourable medium for the electrochemical detection of pyocyanin, and Mg2+ was shown to be the most effective supporting additive ion for the detection. 1-Naphthylamine was shown to be the most effective supporting antibiotic reagent when supplied in the medium to enhancing the presence of pyocyanina compared to the other two antibiotics. The results indicated that the improved selective medium (ISM) can effectively inhibit/limit the growth of various microorganisms other than P. aeruginosa and obviously enhance the production of pyocyanin in environmental samples that contained P. aeruginosa. This study provided a method for simple and fast electrochemical detection of P. aeruginosa in complex microbial samples.
URI: http://hdl.handle.net/10397/79920
EISSN: 1452-3981
DOI: 10.20964/2018.04.20
Rights: © 2018 The Authors. Published by ESG (www.electrochemsci.org). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
The following publication Li, S. H., Mou, Q. Q., Feng, N. Z., & Leung, P. H. M. (2018). A selective medium for pyocyanin-dependent fast electrochemical detection of pseudomonas aeruginosa in environmental microbial samples. International Journal of Electrochemical Science, 13(4), 3789-3798 is available at https://dx.doi.org/10.20964/2018.04.20
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