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Title: Efficient production of secretory Streptomyces clavuligerus beta-lactamase inhibitory protein (BLIP) in Pichia pastoris
Authors: Law, KH 
Tsang, MW 
Wong, YK 
Tsang, MS 
Lau, PY 
Wong, KY 
Ho, KP 
Leung, YC 
Keywords: Beta-Lactamase inhibitor
Beta-Lactamase inhibitory protein
Pichia pastoris
Recombinant protein expression
Secretory protein expression
Issue Date: 2018
Publisher: Springer
Source: AMB Express, 20 Apr. 2018, v. 8, 64, p. 1-11 How to cite?
Journal: AMB Express 
Abstract: beta-Lactamase inhibitory protein (BLIP), a low molecular weight protein from Streptomyces clavuligerus, has a wide range of potential applications in the fields of biotechnology and pharmaceutical industry because of its tight interaction with and potent inhibition on clinically important class A beta-lactamases. To meet the demands for considerable amount of highly pure BLIP, this study aimed at developing an efficient expression system in eukaryotic Pichia pastoris (a methylotrophic yeast) for production of BLIP. With methanol induction, recombinant BLIP was overexpressed in P. pastoris X-33 and secreted into the culture medium. A high yield of similar to 300 mg/L culture secretory BLIP recovered from the culture supernatant without purification was found to be > 90% purity. The recombinant BLIP was fully active and showed an inhibition constant (K-i) for TEM-1 beta-lactamase (0.55 +/- 0.07 nM) comparable to that of the native S. clavuligerus-expressed BLIP (0.5 nM). Yeast-produced BLIP in combination with ampicillin effectively inhibited the growth of beta-lactamase-producing Gram-positive Bacillus. Our approach of expressing secretory BLIP in P. pastoris gave 71- to 1200-fold more BLIP with high purity than the other conventional methods, allowing efficient production of large amount of highly pure BLIP, which merits fundamental science studies, drug development and biotechnological applications.
EISSN: 2191-0855
DOI: 10.1186/s13568-018-0586-3
Rights: © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
The following publication Law, K. H., Tsang, M. W., Wong, Y. K., Tsang, M. S., Lau, P. Y., Wong, K. Y., … & Leung, Y. C. (2018). Efficient production of secretory Streptomyces clavuligerus beta-lactamase inhibitory protein (BLIP) in Pichia pastoris. AMB Express, 8, 64, 1-11 is available at
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