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Title: | The role of the RhoA/ROCK signaling pathway in mechanical strain-induced scleral myofibroblast differentiation | Authors: | Yuan, Y Li, M To, CH Lam, TC Wang, P Yu, YJ Chen, QZ Hu, XJ Ke, BL |
Issue Date: | 2018 | Source: | Investigative ophthalmology and visual science, July 2018, v. 59, no. 8, p. 3619-3629 | Abstract: | PURPOSE. Biomechanical properties changes and alpha-smooth muscle actin (alpha-SMA) overexpression are involved in myopia scleral remodeling. However, interactions between altered tissue biomechanics and cellular signaling that sustain scleral remodeling have not been well defined. We determine the mechanisms of mechanotransduction in the regulation of alpha-SMA expression during myopia scleral remodeling. Methods. Guinea pigs were used to establish a form-deprivation myopia (FDM) model. Protein profiles in myopic sclera were examined using tandem mass spectrometry. Ras homolog gene family member A (RhoA) and alpha-SMA expressions were confirmed using quantitative (q) RTPCR and Western blotting. Scleral fibroblasts were cultured and subjected to 4% cyclic strain. Levels of RhoA, rho-associated protein kinase-2 (ROCK2), myocardin-related transcription factor-A (MRTF-A), serum response factor (SRF), and alpha-SMA were determined by qRT-PCR and Western blotting in groups with or without the RhoA siRNA or ROCK inhibitor Y27632. MRTF-A and alpha-SMA were evaluated by confocal immunofluorescent microscopy and myofibroblasts were enumerated using flow cytometry. Results. mRNA and protein levels of RhoA and alpha-SMA were significantly increased in the FDM eyes after 4 weeks of form-deprivation treatment. The 4% static strain increased expressions of RhoA, ROCK2, MRTF-A, SRF, and alpha-SMA as well as nuclear translocalization of MRTF-A in scleral fibroblasts compared to those without strain stimulation. Additionally, the percentage of myofibroblasts increased after strain stimulation. Conversely, inhibition of RhoA or ROCK2 reversed the strain-induced alpha-SMA expression and myofibroblast ratio. Conclusions. Mechanical strain activated RhoA signaling and scleral myofibroblast differentiation. Strain also mediated myofibroblast differentiation via the RhoA/ROCK2-MRTF-A/SRF pathway. These findings provided evidence for a mechanical strain-induced RhoA/ROCK2 pathway that may contribute to myopia scleral remodeling. |
Keywords: | Form-deprivation myopia Scleral fibroblast Alpha-SMA RhoA/ROCK2 Mechanotransduction |
Publisher: | Association for Research in Vision and Ophthalmology | Journal: | Investigative ophthalmology and visual science | ISSN: | 0146-0404 | EISSN: | 1552-5783 | DOI: | 10.1167/iovs.17-23580 | Rights: | © Copyright 2018 The Authors This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/). The following publication Yuan Y, Li M, To CH, et al. The role of the RhoA/ROCK signaling pathway in mechanical strain-induced scleral myofibroblast differentiation. Invest Ophthalmol Vis Sci. 2018;59:3619–3629 is available at https://doi.org/10.1167/iovs.17-23580 |
Appears in Collections: | Journal/Magazine Article |
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