Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/77976
DC Field | Value | Language |
---|---|---|
dc.contributor | Institute of Textiles and Clothing | - |
dc.creator | Chen, J | - |
dc.creator | Hu, J | - |
dc.creator | Zuo, P | - |
dc.creator | Su, X | - |
dc.creator | Liu, Z | - |
dc.creator | Yang, M | - |
dc.date.accessioned | 2018-08-28T01:35:59Z | - |
dc.date.available | 2018-08-28T01:35:59Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/77976 | - |
dc.language.iso | en | en_US |
dc.publisher | Royal Society of Chemistry | en_US |
dc.rights | © The Royal Society of Chemistry 2018 | en_US |
dc.rights | Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (https://creativecommons.org/licenses/by/3.0/). | en_US |
dc.rights | The following publication Chen, J., Hu, J., Zuo, P., Su, X., Liu, Z., & Yang, M. (2018). Tailor-made spider-eggcase-silk spheres for efficient lysosomal drug delivery. RSC advances, 8(17), 9394-9401 is available at https://doi.org/10.1039/c8ra00232k | en_US |
dc.title | Tailor-made spider-eggcase-silk spheres for efficient lysosomal drug delivery | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 9394 | - |
dc.identifier.epage | 9401 | - |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 17 | - |
dc.identifier.doi | 10.1039/c8ra00232k | - |
dcterms.abstract | Spider silks are attractive biopolymers due to their excellent mechanical properties and biomimetic potential. To optimize the electrostatic interaction for lysosomal drug delivery, a spider-eggcase-silk protein was genetically engineered using 5× His Tag with a tailor-made isoelectric point of 4.8. By a facile HFIP-on-oil method, silk spheres were assembled as rapidly as 10 s. After the post-treatment of ethanol, silk spheres were determined with an improved compressive modulus by AFM indentation. Under incubation of silk spheres in a Doxorubicin solution, a maximum of 35% loading and average of 30% loading efficiency were determined. In the cytotoxicity experiment, silk spheres exhibited intrinsic biocompatibility and showed good control of the loaded drug in the neutral PBS solution. Significantly, by 96 h, the accumulative drug release at pH 4.5 was approximately 4.5-fold higher than that at pH 7.4. By conducting the platelet adhesion and hemolysis assay, Doxorubicin-loaded silk spheres exhibited good hemocompatibility. To further demonstrate this release behavior, within 24 h, Doxorubicin-loaded silk spheres were efficiently delivered to lysosomes and then released the payload to the nuclei of Hela cells. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | RSC advances, 2018, v. 8, no. 17, p. 9394-9401 | - |
dcterms.isPartOf | RSC advances | - |
dcterms.issued | 2018 | - |
dc.identifier.isi | WOS:000431972600044 | - |
dc.identifier.scopus | 2-s2.0-85043365357 | - |
dc.identifier.eissn | 2046-2069 | - |
dc.identifier.rosgroupid | 2017004299 | - |
dc.description.ros | 2017-2018 > Academic research: refereed > Publication in refereed journal | - |
dc.description.validate | 201808 bcrc | - |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Chen_Spider_Eggcase_Silk.pdf | 804.14 kB | Adobe PDF | View/Open |
Page views
101
Last Week
1
1
Last month
Citations as of May 12, 2024
Downloads
37
Citations as of May 12, 2024
SCOPUSTM
Citations
10
Last Week
0
0
Last month
Citations as of May 17, 2024
WEB OF SCIENCETM
Citations
10
Last Week
0
0
Last month
Citations as of May 16, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.