Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/77319
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dc.contributor.authorOudeng, Gen_US
dc.contributor.authorAu, Men_US
dc.contributor.authorShi, Jen_US
dc.contributor.authorWen, Cen_US
dc.contributor.authorYang, Men_US
dc.date.accessioned2018-07-30T08:27:33Z-
dc.date.available2018-07-30T08:27:33Z-
dc.date.issued2018-
dc.identifier.citationACS applied materials and interfaces, 2018, v. 10, no. 1, p. 350-360en_US
dc.identifier.issn1944-8244-
dc.identifier.urihttp://hdl.handle.net/10397/77319-
dc.description.abstractHere, we report the one-step in situ detection of targeted miRNAs expression in single living cancer cells via MoS2 nanosheet-based fluorescence on/off probes. The strategy is based on the folic acid (FA)-poly(ethylene glycol)-functionalized MoS2 nanosheets with adsorbed dye-labeled single-stranded DNA (ssDNA). Once the nanoprobes are internalized into cancer cells, the hybridization between the probes and target miRNA results in the detachment of dye-labeled ssDNA from MoS2 nanosheets surface, leading to the green fluorescence recovery. In this nanoprobe, MoS2 nanosheets offer advantages of high fluorescence quenching efficiency and extremely low toxicity. The FA conjugation could protect the probes and improve cancer cell transfection efficiency. The ability of this nanoprobe for endogenous miRNA detection in single living cancer cells is demonstrated for two types of cancer cells with different miRNA-21 expressions (MCF-7 and Hela cells). This functionalized MoS2 nanosheet-based nanoprobes could provide a sensitive and real-time detection of intracellular miRNA detection platform.en_US
dc.description.sponsorshipDepartment of Biomedical Engineeringen_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofACS applied materials and interfacesen_US
dc.subjectCancer cellsen_US
dc.subjectFluorescence resonance energy transfer (FRET)en_US
dc.subjectIn situ detectionen_US
dc.subjectMiRNAsen_US
dc.subjectMoS2 nanosheetsen_US
dc.titleOne-Step in situ detection of miRNA-21 expression in single cancer cells based on biofunctionalized MoS2 nanosheetsen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage350-
dc.identifier.epage360-
dc.identifier.volume10-
dc.identifier.issue1-
dc.identifier.doi10.1021/acsami.7b18102-
dc.identifier.scopus2-s2.0-85040460001-
dc.identifier.eissn1944-8252-
dc.identifier.rosgroupid2017000559-
dc.description.ros2017-2018 > Academic research: refereed > Publication in refereed journal-
dc.description.validate201807 bcrc-
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