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http://hdl.handle.net/10397/66112
| Title: | Development of a novel quantum dots and graphene oxide based fret assay for rapid detection of inva gene of Salmonella | Authors: | Guo, J Chan, EWC Chen, S Zeng, Z |
Issue Date: | 17-Jan-2017 | Source: | Frontiers in microbiology, 17 Jan. 2017, v. 8, 8, p. 1-7 | Abstract: | A novel, rapid and simple fluorescence resonance energy transfer (FRET) based Salmonella specific gene, invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the Salmonella specific invA gene could be sensitively and specifically detected with a limit of detection (LOD) of ~4 nM of the invA gene in 20 min. The developed system has the potential to be used for Salmonella detection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens. | Keywords: | FRET assay Graphene oxide InvA Quantum dots Rapid detection Salmonella |
Publisher: | Frontiers Research Foundation | Journal: | Frontiers in microbiology | ISSN: | 1664-302X | DOI: | 10.3389/fmicb.2017.00008 | Rights: | Copyright © 2017 Guo, Chan, Chen and Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. The following publication Guo J, Chan EWC, Chen S and Zeng Z (2017) Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella. Front. Microbiol. 8:8,1-7 is available at https://dx.doi.org/10.3389/fmicb.2017.00008 |
| Appears in Collections: | Journal/Magazine Article |
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| Guo_Quantum_Dots_Graphene.pdf | 1.52 MB | Adobe PDF | View/Open |
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