Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/66112
Title: Development of a novel quantum dots and graphene oxide based fret assay for rapid detection of inva gene of Salmonella
Authors: Guo, J
Chan, EWC
Chen, S
Zeng, Z
Keywords: FRET assay
Graphene oxide
InvA
Quantum dots
Rapid detection
Salmonella
Issue Date: 2017
Publisher: Frontiers Research Foundation
Source: Frontiers in microbiology, 2017, v. 8, no. JAN, 8 How to cite?
Journal: Frontiers in microbiology 
Abstract: A novel, rapid and simple fluorescence resonance energy transfer (FRET) based Salmonella specific gene, invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the Salmonella specific invA gene could be sensitively and specifically detected with a limit of detection (LOD) of ~4 nM of the invA gene in 20 min. The developed system has the potential to be used for Salmonella detection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens.
URI: http://hdl.handle.net/10397/66112
ISSN: 1664-302X
DOI: 10.3389/fmicb.2017.00008
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