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Title: Factors influencing the abundance of the side population in a human myeloma cell line
Authors: Mo, SL
Li, J
Loh, YS
Brown, RD
Smith, AL
Chen, Y
Joshua, D
Roufogalis, BD
Li, GQ
Fan, K
Sze, MYD
Issue Date: 2011
Publisher: Hindawi Publishing Corporation
Source: Bone marrow research, v. 2011, 524845, p.1-8 How to cite?
Journal: Bone marrow research 
Abstract: Side population (SP) refers to a group of cells, which is capable to efflux Hoechst 33342, a DNA-binding dye. SP cells exist both in normal and tumor tissues. Although SP abundance has been used as an indicator for disease prognostic and drug screening in many research projects, few studies have systematically examined the factors influencing SP analysis. In this study we aim to develop a more thorough understanding of the multiple factors involved in SP analysis including Hoechst 33342 staining and cell culture. RPMI-8226, a high SP percentage (SP%) human myeloma cell line was employed here. The results showed that SP% was subject to staining conditions including: viable cell proportion, dye concentration, staining cell density, incubation duration, staining volume, and mix interval. In addition, SP% was highest in day one after passage, while dropped steadily over time. This study shows that both staining conditions and culture duration can significantly affect SP%. In this case, any conclusions based on SP% should be interpreted cautiously. The relation between culture duration and SP% suggests that the incidence of SP cells may be related to cell proliferation and cell cycle phase. Maintaining these technical variables consistently is essential in SP research.
ISSN: 2090-2999 (print)
2090-3006 (online)
DOI: 10.1155/2011/524845
Rights: Copyright © 2011 Sui-Lin Mo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Appears in Collections:Journal/Magazine Article

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