Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/65360
Title: A nanoporous alumina membrane based electrochemical biosensor for histamine determination with biofunctionalized magnetic nanoparticles concentration and signal amplification
Authors: Ye, W
Xu, Y
Zheng, L
Zhang, Y
Yang, M 
Sun, P
Keywords: Electrochemical biosensor
Histamine
Magnetic nanoparticles
Nanoporous alumina membrane
Issue Date: 2016
Publisher: Molecular Diversity Preservation International (MDPI)
Source: Sensors, 2016, v. 16, no. 10, 1767 How to cite?
Journal: Sensors 
Abstract: Histamine is an indicator of food quality and indispensable in the efficient functioning of various physiological systems. Rapid and sensitive determination of histamine is urgently needed in food analysis and clinical diagnostics. Traditional histamine detection methods require qualified personnel, need complex operation processes, and are time-consuming. In this study, a biofunctionalized nanoporous alumina membrane based electrochemical biosensor with magnetic nanoparticles (MNPs) concentration and signal amplification was developed for histamine determination. Nanoporous alumina membranes were modified by anti-histamine antibody and integrated into polydimethylsiloxane (PDMS) chambers. The specific antibody modified MNPs were used to concentrate histamine from samples and transferred to the antibody modified nanoporous membrane. The MNPs conjugated to histamine were captured in the nanopores via specific reaction between histamine and anti-histamine antibody, resulting in a blocking effect that was amplified by MNPs in the nanopores. The blockage signals could be measured by electrochemical impedance spectroscopy across the nanoporous alumina membrane. The sensing platform had great sensitivity and the limit of detection (LOD) reached as low as 3 nM. This biosensor could be successfully applied for histamine determination in saury that was stored in frozen conditions for different hours, presenting a potentially novel, sensitive, and specific sensing system for food quality assessment and safety support.
URI: http://hdl.handle.net/10397/65360
EISSN: 1424-8220
DOI: 10.3390/s16101767
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

1
Last Week
0
Last month
Citations as of Sep 11, 2017

WEB OF SCIENCETM
Citations

1
Last Week
0
Last month
Citations as of Sep 6, 2017

Page view(s)

14
Checked on Sep 18, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.