Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/64007
PIRA download icon_1.1View/Download Full Text
Title: Studies on fermentation of synthesizing poly-β - hydroxyalkanoate(pha) by sphaerotilus sp.
Other Title: 球衣菌合成聚羟基烷酸(PHA)的发酵研究
Authors: Yang, YH
Wu, ZH
Yu, PH
Zhong, SQ
Issue Date: 2001
Source: 微生物學通報 (Microbiology), 2001, v. 28, no. 4, p. 61-66
Abstract: 研究了球衣菌 (Sphaerotilussp.)W991 3 6合成聚羟基烷酸 (PHA)的培养基配方及发酵条件。结果表明 ,W991 3 6适宜发酵培养基配方为 :葡萄糖 1 0 2 5g/L ,蛋白胨 2 6 3g/L ,MgSO4·7H2 O 0 1 7g/L ,CaCl2 0 0 5g/L ,NaH2 PO4·2H2 O 0 0 2g/L ,K2 HPO40 0 4g/L ,KH2 PO40 0 3g/L ;最佳接种量为 0 1 3 8g (干 ) / 1 0 0mL ,培养基适宜初始pH为 7 0~ 7 5 ,发酵 3 6h可获得较大的细胞干重与PHA产量 ,经1 H NMR与GC分析 ,其PHA为羟基丁酸与羟基戊酸共聚体P (HB co HV) ,其中羟基戊酸 (HV)含量达 3 9 3 %。
Fermentation of synthesizing Poly-β-hyd roxyalkanoate(PHA)by Sphaeroti lus sp.W99136 were optimized.The result of uniform design test showed that the o ptimized medium composition contained (g/L):glucose 10 25,peptone 2 63,MgSO 4 ·7H 2O 0 17,CaCl 2 0 05,NaH 2PO 4·2H 2O 0 02,K 2HPO 4 0 04,KH 2PO 4 0 03,The optimum seed c ulture volume was 0 138g(CDW)/100mL.With control initial pH in the medium betwe e n 7 0,higher dry cell weight and PHA content were achieved after fermentation f o r 36h 1H-NMR and GC analysis of PHA products from Sphaero tilus sp.W99136 show that the product was P(HB-co-HV).The HV content of P(HB-c o-HV) came to 39 3%.
Keywords: Sphaerotilus
Poly-β-hydroxya lkanoate(PHA) fermentation
Publisher: 科學出版社
Journal: 微生物學通報 (Microbiology) 
ISSN: 0253-2654
Rights: © 2001 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。
© 2001 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes.
Appears in Collections:Journal/Magazine Article

Files in This Item:
File Description SizeFormat 
r05286.pdf198.02 kBAdobe PDFView/Open
Open Access Information
Status open access
File Version Version of Record
Access
View full-text via PolyU eLinks SFX Query
Show full item record

Page views

65
Last Week
3
Last month
Citations as of Mar 24, 2024

Downloads

87
Citations as of Mar 24, 2024

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.