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Title: Trehalose production by permeabilized cells
Other Titles: 用透性化细胞技术合成海藻糖
Authors: Gao, HL
Yuan, QP
Zhou, Y
Qian, ZM
Keywords: Permeabilized cells
Intracellular enzyme
Issue Date: 2004
Publisher: 科學出版社
Source: 微生物學通報 (Microbiology), 2004, v. 31, no. 3, p. 92-96 How to cite?
Journal: 微生物學通報 (Microbiology) 
Abstract: 建立了一种渗透处理微球菌细胞的方法,得到的透性化细胞可多批使用并能长时间保持胞内酶的活力,极大地提高了单位菌体的利用率,降低了成本,为海藻糖实现工业化开辟了新的思路。实验结果表明:菌悬液用5%(v/v)甲苯处理40min,得到的菌体即为透性化细胞,再以10%淀粉液化液为底物进行海藻糖转化实验,转化率可达70%;该透性化细胞至少可连续进行6批酶反应(12h/批),酶活基本保持稳定。
A permeabilization method of Micrococcus cells has been established. The obtained penneabilized cells could be used for several batches and in the mean time the intracellular enzymes still keep high activity. This method will undoubtedly increase the productivity of the cells and cut down the cost; therefore, it will be a promising way in the treha-lose industrialization. The experiment shows: after being treated with 5% (v/v) methylbenzene for 40min, the cells in suspension were converted into permeabilized cells, then the latter acted on 10% liquefied starch to produce trehaloae, the conversion ratio could reach 70%. The penneabilized cells could be used at least for 6 batchs (12h per batch) and the intracellular enzyme activity kept steady.
ISSN: 0253-2654
Rights: © 2004 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。
© 2004 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes.
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