High cholesterol level upregulate the expression of caveolin-1

Abstract

为了研究高胆固醇血症对血管内皮细胞膜上 Caveolin- 1表达的影响。用免疫组织化学的方法检测了高胆固醇血清刺激的培养人脐静脉内皮细胞株 ECV- 30 4细胞以及高脂血症大鼠动脉内皮上 Caveolin- 1表达量的动态变化。发现高胆固醇可上调体内外内皮细胞膜上 Caveolin- 1的表达。在高血脂模型初期 ,Caveolin- 1的表达量与高血脂水平的维持时程成正相关 ;在高胆固醇血症模型后期 ,血管内皮细胞膜上 Caveolin- 1的表达略有下降 To study the influence of hypercholesterolemia with caveolin 1 on the plasmalemma of vascular endothelium,we used the methods of immunohistochemistry to detect the dynamic changes of caveolin 1 in cultured ECV 304 cells which were stimulated high cholesterol serum and the arterial endothelium of hypercholesterolemia rats. It is resulted that high cholesteorol level can upregulate the expression of caveolin 1 both in vitro and in vivo. In the initial stage of hypercholesterolemia model,the expression of caveolin 1 increased as the time of high cholesterol level added,but in the later period it was decreased slightly.