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Title: Transferrin-bound Yb2 uptake by U-87 MG cells and effect of Yb on proliferation of the cells
Other Titles: 转铁蛋白结合镱跨膜转运及镱对U-87 MG细胞增殖的影响
Authors: Wang, K
Du, XL
Katie, SKY
Li, RC
Sun, HZ
Qian, ZM
Keywords: Yb 3+ / Yb 2Tf
Transferrin/transferrin receptor
U-87 MG cells
Issue Date: 2003
Publisher: 中國學術期刊(光盤版)電子雜誌社
Source: 中国病理生理杂志 (Chinese journal of pathophysiology), 2003, v. 19, no. 7, p. 902-904 How to cite?
Journal: 中国病理生理杂志 (Chinese journal of pathophysiology) 
Abstract: 目的: 研究转铁蛋白 /转铁蛋白受体转运系统对转铁蛋白结合镱 (Yb2 Tf)跨膜转运进入神经胶质瘤U- 87MG细胞 ,以及转铁蛋白结合镱和非转铁蛋白结合镱对U - 87MG细胞增殖的影响。
方法: 细胞培养及ICP -MS镱测定法。
结果: 随Yb2 Tf浓度增加 ,细胞镱摄入量增加。当浓度达 2 μmol/L时 ,细胞摄取镱基本达到饱和状态。细胞摄入镱量也随镱: apoTf摩尔比增大而增加 ,当摩尔比达到 1.5时 ,摄入量达到最高水平。 0 .4 μmol/LYb2 Tf可显著抑制U - 87MG细胞增殖 ,而Yb3 +浓度高达 10 μmol对细胞增殖仅有轻微影响。
结论: 转铁蛋白 /转铁蛋白受体介导的膜转运可能是镱跨越U - 87MG细胞的机制之一。转铁蛋白结合Yb3 +可以有效地抑制U - 87MG细胞的增殖。
Aim: To investigate the role of transferrin/transferrin receptor system in transferrin-bound Yb 2 (Yb 2Tf) uptake by U-87 MG cells and the effect of transferrin-bound and -free Yb 2 on proliferation of U-87 MG cells.
Methods: Cell culture and ICP-MS measurement of Yb 2.
Results: Yb 2Tf uptake by U-87 MG cells increased with the concentrations of Yb 2Tf, and reached saturation as the concentration in the incubation medium was raised to about 2 μmol/L. Also, Yb 2 uptake by the cells increased with increase of the mole ratio (Yb 2: apoTf), reaching a maximum at 1.5 mole ratio. Yb 2Tf in 0.4 μmol/L significantly inhibited proliferation of U-87 MG cells, however, 10 μmol/L Yb 3+ had no significant effect on proliferation of the cells.
Conclusion: The uptake of Yb 2 by U-87 MG cells might be mediated by transferrin/transferrin receptor system. Transferrin-bound but not transferrin-free Yb 2 could significantly inhibit proliferation of U-87 MG cells.
ISSN: 1000-4718
Rights: © 2003 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。
© 2003 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes.
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