Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/62604
Title: | Transferrin-bound Yb2 uptake by U-87 MG cells and effect of Yb on proliferation of the cells | Other Title: | 转铁蛋白结合镱跨膜转运及镱对U-87 MG细胞增殖的影响 | Authors: | Wang, K Du, XL Katie, SKY Li, RC Sun, HZ Qian, ZM |
Issue Date: | 2003 | Source: | 中国病理生理杂志 (Chinese journal of pathophysiology), 2003, v. 19, no. 7, p. 902-904 | Abstract: | 目的: 研究转铁蛋白 /转铁蛋白受体转运系统对转铁蛋白结合镱 (Yb2 Tf)跨膜转运进入神经胶质瘤U- 87MG细胞 ,以及转铁蛋白结合镱和非转铁蛋白结合镱对U - 87MG细胞增殖的影响。 方法: 细胞培养及ICP -MS镱测定法。 结果: 随Yb2 Tf浓度增加 ,细胞镱摄入量增加。当浓度达 2 μmol/L时 ,细胞摄取镱基本达到饱和状态。细胞摄入镱量也随镱: apoTf摩尔比增大而增加 ,当摩尔比达到 1.5时 ,摄入量达到最高水平。 0 .4 μmol/LYb2 Tf可显著抑制U - 87MG细胞增殖 ,而Yb3 +浓度高达 10 μmol对细胞增殖仅有轻微影响。 结论: 转铁蛋白 /转铁蛋白受体介导的膜转运可能是镱跨越U - 87MG细胞的机制之一。转铁蛋白结合Yb3 +可以有效地抑制U - 87MG细胞的增殖。 Aim: To investigate the role of transferrin/transferrin receptor system in transferrin-bound Yb 2 (Yb 2Tf) uptake by U-87 MG cells and the effect of transferrin-bound and -free Yb 2 on proliferation of U-87 MG cells. Methods: Cell culture and ICP-MS measurement of Yb 2. Results: Yb 2Tf uptake by U-87 MG cells increased with the concentrations of Yb 2Tf, and reached saturation as the concentration in the incubation medium was raised to about 2 μmol/L. Also, Yb 2 uptake by the cells increased with increase of the mole ratio (Yb 2: apoTf), reaching a maximum at 1.5 mole ratio. Yb 2Tf in 0.4 μmol/L significantly inhibited proliferation of U-87 MG cells, however, 10 μmol/L Yb 3+ had no significant effect on proliferation of the cells. Conclusion: The uptake of Yb 2 by U-87 MG cells might be mediated by transferrin/transferrin receptor system. Transferrin-bound but not transferrin-free Yb 2 could significantly inhibit proliferation of U-87 MG cells. |
Keywords: | Yb 3+ / Yb 2Tf Transferrin/transferrin receptor Glioma U-87 MG cells |
Publisher: | 中國學術期刊(光盤版)電子雜誌社 | Journal: | 中国病理生理杂志 (Chinese journal of pathophysiology) | ISSN: | 1000-4718 | Rights: | © 2003 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。 © 2003 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes. |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
r19897.pdf | 115.92 kB | Adobe PDF | View/Open |
Page views
118
Last Week
2
2
Last month
Citations as of Mar 24, 2024
Downloads
42
Citations as of Mar 24, 2024
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.