Ormosils-PVA BOD sensing film based on oxygen luminescence quenching

Abstract

通过有机改性溶胶凝胶PVA包埋方法固定微生物菌株 ,以氧光化学传感器作为二次传感 ,制备了响应良好的光化学BOD微生物传感器 .在此基础上 ,考察了微生物传感膜的活化浓度、温度、pH、无机盐及部分金属离子对传感器响应的影响 ,并对传感器的重现性、储存稳定性和线性范围进行了考察 ,实验结果表明BOD含量在 0— 10 0mg L浓度范围内与传感器的荧光值成线性关系 ,传感膜的响应活性保持较好 ,具有较长的使用寿命和良好的稳定性。 An organically modified silicate (ormosil)-PVA as an immobilization material for Hansennula anomala was investigated to develop an optochemical BOD sensor for the determination of BOD. After preconditioning, the BOD sensing film can be steadily used for two months. The linear fluctuant coefficient(R 2) in the ranges of 0—100 mg/L and 0—40 mg/L was 0.9950 and 0.9604, respectively. The effects of activated concentration, temperature, pH, sodium chloride concentration and coexisting metal ions to BOD determination were investigated.