Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/62271
Title: miR-541 contributes to microcystin-LR-induced reproductive toxicity through regulating the expression of p15 in mice
Authors: Meng, X
Zhang, L
Chen, X
Zou, X 
Li, D
Han, X
Keywords: Apoptosis
Male reproduction
MC-LR
MiR-541
P15
Issue Date: 2016
Publisher: Molecular Diversity Preservation International (MDPI)
Source: Toxins, 2016, v. 8, no. 9, 260, p.1-16 How to cite?
Journal: Toxins 
Abstract: Microcystin-leucine arginine (MC-LR) is a harmful cyanotoxin produced by cyanobacteria. MC-LR can exert endocrine-disrupting activities inmany organisms.We have previously demonstrated that MC-LR exerts both acute and chronic reproductive toxicity in male mice, resulting in a decline in sperm quality and damage to testicular structure. Moreover, we also observed extensive alterations in a panel of microRNAs in spermatogonial cells after exposure to MC-LR. In this study, we have confirmed that miR-541 was significantly increased both in GC-1 cells (in vitro) and in mouse testes (in vivo) after exposure to MC-LR. Our data support that p15 was the target gene of miR-541. Increase in miR-541 led to a reduction of p15 and murine double minute2 (MDM2), promoting the activation of p53 signaling and MC-LR-mediated cell apoptosis. Moreover, cells responded to MC-LR with reduced viability and increased apoptosis. Consistently, inhibiting miR-541 could upregulate the expression of p15 and MDM2, resulting in the downregulation of phospho-p53. Downregulation of miR-541 promoted cell viability by reducing MC-LR-induced cell apoptosis. In conclusion, we demonstrate here a crucial role for miR-541 in MC-LR-induced toxic effects on the reproductive system, in an attempt to provide a rational strategy for the diagnosis and treatment of MC-LR-induced impairment in the reproductive system.
URI: http://hdl.handle.net/10397/62271
ISSN: 2072-6651
DOI: 10.3390/toxins8090260
Rights: © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
The following publication Meng, X., Zhang, L., Chen, X., Xiang, Z., Li, D., & Han, X. (2016). miR-541 contributes to Microcystin-LR-induced reproductive toxicity through regulating the expression of p15 in mice. Toxins, 8(9), 260 is available at http://dx.doi.org/10.3390/toxins8090260
Appears in Collections:Journal/Magazine Article

Files in This Item:
File Description SizeFormat 
toxins-08-00260.pdf6.21 MBAdobe PDFView/Open
Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

3
Last Week
0
Last month
Citations as of Aug 13, 2017

WEB OF SCIENCETM
Citations

2
Last Week
0
Last month
Citations as of Aug 14, 2017

Page view(s)

59
Last Week
37
Last month
Checked on Aug 13, 2017

Download(s)

10
Checked on Aug 13, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.