Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/62271
Title: | miR-541 contributes to microcystin-LR-induced reproductive toxicity through regulating the expression of p15 in mice | Authors: | Meng, X Zhang, L Chen, X Zou, X Li, D Han, X |
Issue Date: | 2016 | Source: | Toxins, 2016, v. 8, no. 9, 260, p.1-16 | Abstract: | Microcystin-leucine arginine (MC-LR) is a harmful cyanotoxin produced by cyanobacteria. MC-LR can exert endocrine-disrupting activities inmany organisms.We have previously demonstrated that MC-LR exerts both acute and chronic reproductive toxicity in male mice, resulting in a decline in sperm quality and damage to testicular structure. Moreover, we also observed extensive alterations in a panel of microRNAs in spermatogonial cells after exposure to MC-LR. In this study, we have confirmed that miR-541 was significantly increased both in GC-1 cells (in vitro) and in mouse testes (in vivo) after exposure to MC-LR. Our data support that p15 was the target gene of miR-541. Increase in miR-541 led to a reduction of p15 and murine double minute2 (MDM2), promoting the activation of p53 signaling and MC-LR-mediated cell apoptosis. Moreover, cells responded to MC-LR with reduced viability and increased apoptosis. Consistently, inhibiting miR-541 could upregulate the expression of p15 and MDM2, resulting in the downregulation of phospho-p53. Downregulation of miR-541 promoted cell viability by reducing MC-LR-induced cell apoptosis. In conclusion, we demonstrate here a crucial role for miR-541 in MC-LR-induced toxic effects on the reproductive system, in an attempt to provide a rational strategy for the diagnosis and treatment of MC-LR-induced impairment in the reproductive system. | Keywords: | Apoptosis Male reproduction MC-LR MiR-541 P15 |
Publisher: | Molecular Diversity Preservation International (MDPI) | Journal: | Toxins | ISSN: | 2072-6651 | DOI: | 10.3390/toxins8090260 | Rights: | © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). The following publication Meng, X., Zhang, L., Chen, X., Xiang, Z., Li, D., & Han, X. (2016). miR-541 contributes to Microcystin-LR-induced reproductive toxicity through regulating the expression of p15 in mice. Toxins, 8(9), 260 is available at http://dx.doi.org/10.3390/toxins8090260 |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
toxins-08-00260.pdf | 6.21 MB | Adobe PDF | View/Open |
Page views
122
Last Week
0
0
Last month
Citations as of Apr 14, 2024
Downloads
112
Citations as of Apr 14, 2024
SCOPUSTM
Citations
15
Last Week
0
0
Last month
Citations as of Apr 19, 2024
WEB OF SCIENCETM
Citations
13
Last Week
1
1
Last month
Citations as of Apr 18, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.