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http://hdl.handle.net/10397/61853
Title: | Rationalisation and validation of an acrylamide-free procedure in three-dimensional histological imaging | Authors: | Lai, HM Liu, AKL Ng, WL DeFelice, J Lee, WS Li, H Li, W Ng, HM Chang, RCC Lin, B Wu, W Gentleman, SM |
Issue Date: | 2016 | Source: | PLoS one, 2016, v. 11, no. 6, e0158628 | Abstract: | Three-dimensional visualization of intact tissues is now being achieved by turning tissues transparent. CLARITY is a unique tissue clearing technique, which features the use of detergents to remove lipids from fixed tissues to achieve optical transparency. To preserve tissue integrity, an acrylamide-based hydrogel has been proposed to embed the tissue. In this study, we examined the rationale behind the use of acrylamide in CLARITY, and presented evidence to suggest that the omission of acrylamide-hydrogel embedding in CLAR-ITY does not alter the preservation of tissue morphology and molecular information in fixed tissues. We therefore propose a novel and simplified workflow for formaldehyde-fixed tissue clearing, which will facilitate the laboratory implementation of this technique. Furthermore, we have investigated the basic tissue clearing process in detail and have highlighted some areas for targeted improvement of technologies essential for the emerging subject of threedimensional histology. | Publisher: | Public Library of Science | Journal: | PLoS one | EISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0158628 | Rights: | © 2016 Lai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The following publication: Lai HM, Liu AKL, Ng W-L, DeFelice J, Lee WS, Li H, et al. (2016) Rationalisation and Validation of an Acrylamide-Free Procedure in Three-Dimensional Histological Imaging. PLoS ONE 11(6): e0158628 is available at https://doi.org/10.1371/journal.pone.0158628 |
Appears in Collections: | Journal/Magazine Article |
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