Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/61853
Title: Rationalisation and validation of an acrylamide-free procedure in three-dimensional histological imaging
Authors: Lai, HM
Liu, AKL
Ng, WL
DeFelice, J
Lee, WS
Li, H
Li, W
Ng, HM
Chang, RCC
Lin, B 
Wu, W
Gentleman, SM
Issue Date: 2016
Publisher: Public Library of Science
Source: PLoS one, 2016, v. 11, no. 6, e0158628 How to cite?
Journal: PLoS one 
Abstract: Three-dimensional visualization of intact tissues is now being achieved by turning tissues transparent. CLARITY is a unique tissue clearing technique, which features the use of detergents to remove lipids from fixed tissues to achieve optical transparency. To preserve tissue integrity, an acrylamide-based hydrogel has been proposed to embed the tissue. In this study, we examined the rationale behind the use of acrylamide in CLARITY, and presented evidence to suggest that the omission of acrylamide-hydrogel embedding in CLAR-ITY does not alter the preservation of tissue morphology and molecular information in fixed tissues. We therefore propose a novel and simplified workflow for formaldehyde-fixed tissue clearing, which will facilitate the laboratory implementation of this technique. Furthermore, we have investigated the basic tissue clearing process in detail and have highlighted some areas for targeted improvement of technologies essential for the emerging subject of threedimensional histology.
URI: http://hdl.handle.net/10397/61853
EISSN: 1932-6203
DOI: 10.1371/journal.pone.0158628
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