Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/5833
Title: Investigation of the biosynthetic potential of endophytes in traditional Chinese anticancer herbs
Authors: Miller, KI
Qing, C
Sze, MYD
Neilan, BA
Keywords: Polyketide synthase genes
Nonribosomal peptide synthetases
Plant antitumor agents
Fungal endophytes
Natural-products
Antimutagenic activity
Future-prospects
Antibiotics
NRPS
Inhibitor
Issue Date: 22-May-2012
Publisher: Public Library of Science
Source: PLoS one, 22 May, 2012, v. 7, no. 5, e35953, p. 1-12 How to cite?
Journal: PLoS one 
Abstract: Traditional Chinese medicine encompasses a rich empirical knowledge of the use of plants for the treatment of disease. In addition, the microorganisms associated with medicinal plants are also of interest as the producers of the compounds responsible for the observed plant bioactivity. The present study has pioneered the use of genetic screening to assess the potential of endophytes to synthesize bioactive compounds, as indicated by the presence of non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) genes. The total DNA extracts of 30 traditional Chinese herbs, were screened for functional genes involved in the biosynthesis of bioactive compounds. The four PCR screens were successful in targeting four bacterial PKS, six bacterial NRPS, ten fungal PKS and three fungal NRPS gene fragments. Analysis of the detected endophyte gene fragments afforded consideration of the possible bioactivity of the natural products produced by endophytes in medicinal herbs. This investigation describes a rapid method for the initial screening of medicinal herbs and has highlighted a subset of those plants that host endophytes with biosynthetic potential. These selected plants can be the focus of more comprehensive endophyte isolation and natural product studies.
URI: http://hdl.handle.net/10397/5833
EISSN: 1932-6203
DOI: 10.1371/journal.pone.0035953
Rights: © 2012 Miller et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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