Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/5832
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Du, F | - |
dc.creator | Qian, ZM | - |
dc.creator | Zhu, L | - |
dc.creator | Wu, XM | - |
dc.creator | Yung, WH | - |
dc.creator | Tsim, TY | - |
dc.creator | Ke, Y | - |
dc.date.accessioned | 2014-12-11T08:23:24Z | - |
dc.date.available | 2014-12-11T08:23:24Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/5832 | - |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | © 2009 Du et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.subject | Divalent metal transporter-1 | en_US |
dc.subject | Cultured rat astrocytes | en_US |
dc.subject | Parkinsons-disease | en_US |
dc.subject | Induced dyskinesia | en_US |
dc.subject | Transferrin-free | en_US |
dc.subject | Reactive oxygen | en_US |
dc.subject | Plasma-membrane | en_US |
dc.subject | Iron uptake | en_US |
dc.subject | Brain | en_US |
dc.subject | Neurons | en_US |
dc.title | L-DOPA neurotoxicity is mediated by up-regulation of DMT1 - IRE expression | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 12 | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 2 | - |
dc.identifier.doi | 10.1371/journal.pone.0004593 | - |
dcterms.abstract | Background: The mechanisms underlying neurotoxicity caused by L-DOPA are not yet completely known. Based on recent findings, we speculated that the increased expression of divalent metal transporter 1 without iron-response element (DMT1-IRE) induced by L-DOPA might play a critical role in the development of L-DOPA neurotoxicity. To test this hypothesis, we investigated the effects of astrocyte-conditioned medium (ACM) and siRNA DMT-IRE on L-DOPA neurotoxicity in cortical neurons. | - |
dcterms.abstract | Methods and Findings: We demonstrated that neurons treated with L-DOPA have a significant dose-dependent decrease in neuronal viability (MTT Assay) and increase in iron content (using a graphite furnace atomic absorption spectrophotometer), DMT1-IRE expression (Western blot analysis) and ferrous iron (55Fe(II)) uptake. Neurons incubated in ACM with or without L-DOPA had no significant differences in their morphology, Hoechst-33342 staining or viability. Also, ACM significantly inhibited the effects of L-DOPA on neuronal iron content as well as DMT1-IRE expression. In addition, we demonstrated that infection of neurons with siRNA DMT-IRE led to a significant decrease in DMT1-IRE expression as well as L-DOPA neurotoxicity. | - |
dcterms.abstract | Conclusion:The up-regulation of DMT1-IRE and the increase in DMT1-IRE-mediated iron influx play a key role in L-DOPA neurotoxicity in cortical neurons. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | PLoS one, 25 Feb., 2009, v. 4, no. 2, e4593, p. 1-12 | - |
dcterms.isPartOf | PLoS one | - |
dcterms.issued | 2009-02-25 | - |
dc.identifier.isi | WOS:000278086700010 | - |
dc.identifier.scopus | 2-s2.0-62349111542 | - |
dc.identifier.pmid | 19240805 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.rosgroupid | r41212 | - |
dc.description.ros | 2008-2009 > Academic research: refereed > Publication in refereed journal | - |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
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Du_L-DOPA_Neurotoxicity_DMT1.pdf | 4.67 MB | Adobe PDF | View/Open |
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