Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/55486
Title: High Resolution Quantitative Angle-Scanning Widefield Surface Plasmon Microscopy
Authors: Tan, HM
Pechprasarn, S
Zhang, J
Pitter, MC
Somekh, MG 
Issue Date: 2016
Publisher: Nature Publishing Group
Source: Scientific reports, 2016, v. 6, 20195 How to cite?
Journal: Scientific reports 
Abstract: We describe the construction of a prismless widefield surface plasmon microscope; this has been applied to imaging of the interactions of protein and antibodies in aqueous media. The illumination angle of spatially incoherent diffuse laser illumination was controlled with an amplitude spatial light modulator placed in a conjugate back focal plane to allow dynamic control of the illumination angle. Quantitative surface plasmon microscopy images with high spatial resolution were acquired by post-processing a series of images obtained as a function of illumination angle. Experimental results are presented showing spatially and temporally resolved binding of a protein to a ligand. We also show theoretical results calculated by vector diffraction theory that accurately predict the response of the microscope on a spatially varying sample thus allowing proper quantification and interpretation of the experimental results.
URI: http://hdl.handle.net/10397/55486
EISSN: 2045-2322
DOI: 10.1038/srep20195
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

Page view(s)

27
Last Week
4
Last month
Checked on Aug 21, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.