Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/55486
Title: | High Resolution Quantitative Angle-Scanning Widefield Surface Plasmon Microscopy | Authors: | Tan, HM Pechprasarn, S Zhang, J Pitter, MC Somekh, MG |
Issue Date: | 2016 | Source: | Scientific reports, 1 2016, v. 6, no. , p. 1-11 | Abstract: | We describe the construction of a prismless widefield surface plasmon microscope; this has been applied to imaging of the interactions of protein and antibodies in aqueous media. The illumination angle of spatially incoherent diffuse laser illumination was controlled with an amplitude spatial light modulator placed in a conjugate back focal plane to allow dynamic control of the illumination angle. Quantitative surface plasmon microscopy images with high spatial resolution were acquired by post-processing a series of images obtained as a function of illumination angle. Experimental results are presented showing spatially and temporally resolved binding of a protein to a ligand. We also show theoretical results calculated by vector diffraction theory that accurately predict the response of the microscope on a spatially varying sample thus allowing proper quantification and interpretation of the experimental results. | Publisher: | Nature Publishing Group | Journal: | Scientific reports | EISSN: | 2045-2322 | DOI: | 10.1038/srep20195 | Rights: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The following publication Tan, H., Pechprasarn, S., Zhang, J. et al. High Resolution Quantitative Angle-Scanning Widefield Surface Plasmon Microscopy. Sci Rep 6, 20195 (2016) is available at https://dx.doi.org/10.1038/srep20195 |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Tan_Quantitative_Angle-Scanning_Widefield.pdf | 4.29 MB | Adobe PDF | View/Open |
Page views
106
Last Week
2
2
Last month
Citations as of Apr 21, 2024
Downloads
53
Citations as of Apr 21, 2024
SCOPUSTM
Citations
26
Last Week
0
0
Last month
Citations as of Apr 19, 2024
WEB OF SCIENCETM
Citations
22
Last Week
0
0
Last month
Citations as of Apr 25, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.